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ST1705 Anti-TOMM20 Mouse mAb (4F3)

This Anti-TOMM20 Mouse mAb (4F3) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TOMM20.

Anti-TOMM20 Mouse mAb (4F3): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: Anti-Translocase of Outer Mitochondrial Membrane 20

ST1705
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
ST1705-100UG 100 μg
Description
OverviewRecognizes the ~12-16 kDa TOMM20 protein in HeLa, PC12, and NIH3T3 cells and human small intestine tissue.
Catalogue NumberST1705
Brand Family Calbiochem®
SynonymsAnti-Translocase of Outer Mitochondrial Membrane 20
Application Data
Detection of human TOMM20 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (1-5 µg/ml). Detection: chemiluminescence.

Detection of rat TOMM20 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse TOMM20 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human TOMM20 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (10 µg/ml). Detection: fluorescence.

Detection of human TOMM20 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (3 µg/ml). Detection: colorimetric.
References
Product Information
Formliquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlHeLa cells, PC12 cells, NIH3T3 cells, Human small intestine tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human TOMM20 (aa 1-146), expressed as a GST fusion protein
Clone4F3
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
ST1705-100UG 04055977207347

Documentation

Anti-TOMM20 Mouse mAb (4F3) Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-November-2010 JLN
SynonymsAnti-Translocase of Outer Mitochondrial Membrane 20
ApplicationELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human TOMM20 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (1-5 µg/ml). Detection: chemiluminescence.

Detection of rat TOMM20 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse TOMM20 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human TOMM20 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (10 µg/ml). Detection: fluorescence.

Detection of human TOMM20 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (3 µg/ml). Detection: colorimetric.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~12-16 kDa TOMM20 protein.
BackgroundThe TOMM20 protein is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 , it functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.
HostMouse
ImmunogenFull-length, recombinant, human TOMM20 (aa 1-146), expressed as a GST fusion protein
Clone4F3
IsotypeIgG₁
Specieshuman, mouse, rat
Positive controlHeLa cells, PC12 cells, NIH3T3 cells, Human small intestine tissue
Negative control293T cells
Formliquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review