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AP1159 Anti-TNFRSF14 Mouse mAb (2G6-2C7)

This Anti-TNFRSF14 Mouse mAb (2G6-2C7) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of TNFRSF14.

Anti-TNFRSF14 Mouse mAb (2G6-2C7): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: Anti-Tumor Necrosis Factor Receptor Super family 14

AP1159
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
AP1159-100UG 100 μg
Description
OverviewRecognizes the TNFRSF14 protein in human pancreatic cancer tissue.
Catalogue NumberAP1159
Brand Family Calbiochem®
SynonymsAnti-Tumor Necrosis Factor Receptor Super family 14
Application Data
Detection of human TNFRSF14 by immunoblotting. Sample: Whole cell lysate from 293T cells, transfected with full-length, recombinant, human TNFRSF14 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-TNFRSF14 Mouse mAb (2G6-2C7) (Cat. No. AP1159) (1-5 µg/ml). Detection: Chemiluminescence.

Detection of human TNFRSF14 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human pancreatic cancer tissue. Primary antibody: Anti-TNFRSF14 Mouse mAb (2G6-2C7) (Cat. No. AP1159) (1 µg/ml). Detection: coloritmetric (DAB).
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlHuman pancreatic cancer tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg)
Paraffin Sections (1 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human TNFRSF14 (aa 38-248), expressed as a GST fusion protein
Clone2G6-2C7
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
AP1159-100UG 04055977212655

Documentation

Anti-TNFRSF14 Mouse mAb (2G6-2C7) Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-October-2010 RFH
SynonymsAnti-Tumor Necrosis Factor Receptor Super family 14
ApplicationELISA (see comments)
Immunoblotting (1-5 µg)
Paraffin Sections (1 µg/ml)
Application Data
Detection of human TNFRSF14 by immunoblotting. Sample: Whole cell lysate from 293T cells, transfected with full-length, recombinant, human TNFRSF14 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-TNFRSF14 Mouse mAb (2G6-2C7) (Cat. No. AP1159) (1-5 µg/ml). Detection: Chemiluminescence.

Detection of human TNFRSF14 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human pancreatic cancer tissue. Primary antibody: Anti-TNFRSF14 Mouse mAb (2G6-2C7) (Cat. No. AP1159) (1 µg/ml). Detection: coloritmetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the TNFRSF14 protein.
BackgroundTNFRSF14 is a member of the TNF-receptor superfamily. This receptor was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism. The cytoplasmic region of this receptor was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response.
HostMouse
ImmunogenFull-length, recombinant, human TNFRSF14 (aa 38-248), expressed as a GST fusion protein
Clone2G6-2C7
IsotypeIgG₁
Specieshuman
Positive controlHuman pancreatic cancer tissue
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review