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MAB5258-I Anti-Synaptophysin Antibody, clone SY38

Anti-Synaptophysin, clone SY38, Cat. No. MAB5258-I, is a highly specific mouse monoclonal antibody that targets Synaptophysin and has been tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Anti-Synaptophysin Antibody, clone SY38: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Neuroscience Gene Symbol: SYP UniProt Number: P20488
MAB5258-I
100 µL  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, B, H, RIH(P), WB, IF, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB5258-I
DescriptionAnti-Synaptophysin Antibody, clone SY38
Alternate Names
  • Synaptophysin
  • Major synaptic vesicle protein p38
Background InformationSynaptophysin (UniProt P20488; also known as Major synaptic vesicle protein p38) is encoded by the SYP gene (Gene ID 280937) in bovine species. Synaptophysin is a 38 kDa four-transmembrane glycoprotein on synaptic vesicle (SV) with both its N- and C-termini exposed cytoplasmically. Synaptophysin forms homomultimers in the SV membrane and is heavily phosphorylated on tyrosine residues in its C-terminal pentapeptide repeats. Synapthophysin is also known to interact with SV v-SNARE protein synaptobrevin II (sybII) when sybII is not engaged with other SNARE proteins, suggesting a role of synaptophysin in sybII retrieval during SV endocytosis. Consistently, synaptophysin-knockout neurons display reduced level of sybII at their nerve terminals.
References
Product Information
FormatPurified
PresentationPurified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Synaptophysin, clone SY38, Cat. No. MAB5258-I, is a highly specific mouse monoclonal antibody that targets Synaptophysin and has been tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
  • Immunofluorescence
  • Immunocytochemistry
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Synaptophysin in human cerebellum and cerebral cortex tissue sections.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Synaptophysin in 10 µg of rat hippocampus and human whole brain tissue lysates.

Immunocytochemistry Analysis: Representative lots immunostained presynaptic vesicles of 4% formaldehyde-fxied, 0.2% Triton X-100-permeabilzied primary mouse hippocampal neurons by fluorescent immunocytochemistry (Hu, X., et al. (2008). J. Neurosci. 28(49):13094-13105; Tarr, P.T., and Edwards, P.A. (2008). J. Lipid Res. 49(1):169-182).

Immunofluorescence Analysis: A representative lot immunostained presynaptic membrane of neurons by fluorescent immunohistochemistry staining of 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized, OCT-embedded rat spinal cord cryosections (Stück, E.D., et al. (2012). Neural Plast. 2012:261345).

Immunofluorescence Analysis: A representative lot immunostained synaptophysin around a large neuron in the ventral horn of the lumbar (L3/L4) segment by fluorescent immunohistochemistry staining of 2% paraformaldehyde/0.2% parabenzoquinone-fixed free-floating rat spinal cord sections (Macias, M., et al. (2009). BMC Neurosci. 10:144).

Immunofluorescence Analysis: A representative lot immunostained synaptophysin in bovine (pancreas) and human (pancreas, pheochromocytoma, and islet-cell carcinoma) frozen tissue sections (Wiedenmann, B., et al. (1986). Proc. Natl. Acad. Sci. U.S.A. 83(10):3500-3504).

Western Blotting Analysis: A representative lot detected an upregulated synaptophysin expression in human iPSCs with a 4-bp deletion in DISC1 gene (Wen, Z., et al. (2014). Nature. 515(7527):414-418).

Western Blotting Analysis: A representative lot detected synaptophysin distribution among PC12 rat pheochromocytoma cell membrane fractions (Salazar, G., et al. (2005). Mol. Biol. Cell. 16(8):3692-3704).

Western Blotting Analysis: A representative lot detected only synaptophysin recombinant contructs that contained the flexible segment -SGGGG- in the center of the c-terminal pentapeptide repeats (Knaus, P., and Betz, H. (1990). FEBS Lett. 261(2):358-360).
Biological Information
ImmunogenPresynaptic vesicles from bovine brain (Wiedenmann, B., and Franke, W.W. (1985). Cell. 41(3):1017-1028).
CloneSY38
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone SY38 detects synaptophysin and stains synaptophysin-containing vesicles by targeting the flexible segment -SGGGG- in the center of the C-termimal pentapeptide repeats (Knaus, P., and Betz, H. (1990). FEBS Lett. 261(2):358-360).
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Bovine
  • Human
  • Rat
Species Reactivity NoteBovine, Human, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SYP
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~38 kDa observed. 33.91/34.03/33.31 kDa (bovine/mouse/rat), 33.85/20.76 kDa (human isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse hypothalamus tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Synaptophysin in 10 µg of mouse hypothalamus tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MAB5258-I 04054839092077

Documentation

Anti-Synaptophysin Antibody, clone SY38 Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-Synaptophysin Antibody, clone SY38 Certificados de análisis

CargoNúmero de lote
Anti-Synaptophysin, clone SY38 - 3226282 3226282
Anti-Synaptophysin, clone SY38 - 3314244 3314244
Anti-Synaptophysin, clone SY38 - 3475628 3475628
Anti-Synaptophysin, clone SY38 - 3554706 3554706
Anti-Synaptophysin, clone SY38 - 3861334 3861334
Anti-Synaptophysin, clone SY38 - 3937800 3937800
Anti-Synaptophysin, clone SY38 - 4095086 4095086
Anti-Synaptophysin, clone SY38 - 4151746 4151746
Anti-Synaptophysin, clone SY38 -2814664 2814664
Anti-Synaptophysin, clone SY38 -2823602 2823602
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