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AB125 Anti-Serotonin Antibody

AB125
100 µL  
Purchase on Sigma-Aldrich

Ofertas especiales

Descripción

Replacement Information

Ofertas especiales

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
RIHCRbSerumPolyclonal Antibody
Description
Catalogue NumberAB125
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Serotonin Antibody
Alternate Names
  • 5-HT
References
Product Information
FormatSerum
HS Code3002 15 90
PresentationRabbit antiserum. Liquid with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Serotonin Antibody detects level of Serotonin & has been published & validated for use in IH.
Key Applications
  • Immunohistochemistry
Application NotesImmunohistochemistry: 1:1,000-1:2,500 by ABC or enhanced detection. Glutaraldehyde fixation is required. Antibody will not react with non-glutaraldehyde treated tissues. Blocking should be with serum or BSA containing 0.1-0.2% triton X-100; remove all blocking an detergents during primary antibody incubation. Dilute only in PBS or TBS for best results. For fluorescent detection antibody dilution must be reduced, but it is not recommended because of glutaraldehyde autofluorescence.



Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen5-Hydroxytryptamine-glutaraldehyde-Poly-lysine
HostRabbit
SpecificitySerotonin (5-Hydroxytryptamine)

The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:



Compound Cross-reactivity

5-Hydroxytryptamine-G-PL 1

5-Methoxytryptamine-G-PL 1/25

Tryptamine-G-PL 1/60

5-Hydroxytryptophan-G-PL 1/175

5-Hydroxytryptamine 1/1150

Tryptophan-G-PL 1/3400

5-Methoxytryptophan-G-PL 1/19800



The antisera was also tested for specificity using the free-floating PAP technique on rat

raphe nuclei.



Abbreviations:



(G) Glutaraldehyde

(=) Non-reduced conjugate

(PL) Poly-lysine
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
AB125 04053252274589

Documentation

Anti-Serotonin Antibody Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-Serotonin Antibody Certificados de análisis

CargoNúmero de lote
RABBIT ANTI-SEROTONIN POLYCLONAL ANTIBODY - 2148207 2148207
RABBIT ANTI-SEROTONIN POLYCLONAL ANTIBODY - 2387460 2387460
RABBIT ANTI-SEROTONIN - 3188676 3188676
RABBIT ANTI-SEROTONIN - 3434578 3434578
RABBIT ANTI-SEROTONIN - 3660445 3660445
RABBIT ANTI-SEROTONIN -2586500 2586500
RABBIT ANTI-SEROTONIN -2761648 2761648
RABBIT ANTI-SEROTONIN POLYCLONAL ANTIBODY 2896348
RABBIT ANTI-SEROTONIN POLYCLONAL ANTIBODY 2884710
RABBIT ANTI-SEROTONIN POLYCLONAL ANTIBODY 3091943

Referencias bibliográficas

Visión general referenciasAplicación EspeciePub Med ID
A new tumorsphere culture condition restores potentials of self-renewal and metastasis of primary neuroblastoma in a mouse neuroblastoma model.
Cao, D; Kishida, S; Huang, P; Mu, P; Tsubota, S; Mizuno, M; Kadomatsu, K
PloS one  9  e86813  2014

Mostrar resumen
24466252 24466252
Local potentiation of excitatory synapses by serotonin and its alteration in rodent models of depression.
Cai, X; Kallarackal, AJ; Kvarta, MD; Goluskin, S; Gaylor, K; Bailey, AM; Lee, HK; Huganir, RL; Thompson, SM
Nature neuroscience  16  464-72  2013

Mostrar resumen
23502536 23502536
Time-course gait analysis of hemiparkinsonian rats following 6-hydroxydopamine lesion.
Hsieh TH, Chen JJ, Chen LH, Chiang PT, Lee HY
Behav Brain Res  222  1-9. [Epub ahead of print]  2010

Mostrar resumen
21435355 21435355
The chemical code of porcine enteric neurons and the number of enteric glial cells are altered by dietary probiotics.
A di Giancamillo,F Vitari,G Bosi,G Savoini,C Domeneghini
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society  22  2009

Mostrar resumen
20524986 20524986
Food-reward signalling in the suprachiasmatic clock.
Jorge Mendoza,Daniel Clesse,Paul Pévet,Etienne Challet
Journal of neurochemistry  112  2009

Mostrar resumen
20067576 20067576
Immunohistochemical detection of L-DOPA-derived dopamine within serotonergic fibers in the striatum and the substantia nigra pars reticulata in Parkinsonian model rats.
Hiromasa Yamada, Yoshinari Aimi, Ikuko Nagatsu, Kousuke Taki, Motoi Kudo, Ryohachi Arai
Neuroscience research  59  1-7  2007

Mostrar resumen
17586078 17586078
Identification of wake-active dopaminergic neurons in the ventral periaqueductal gray matter.
Lu, J; Jhou, TC; Saper, CB
The Journal of neuroscience : the official journal of the Society for Neuroscience  26  193-202  2005

Mostrar resumen
ImmunohistochemistryRat16399687 16399687
The co-existence of biogenic amines and neuropeptides in the type I cells of the cat carotid body.
Wang, Z. et al.
Neuroscience, 47(2):473-480 (1992)  1992

1379355 1379355
Serotonin-like immunoreactivity in Merkel cells and their afferent neurons in touch domes from the hairy skin of rats.
K B English, Z Z Wang, N Stayner, L J Stensaas, H Martin, R P Tuckett
The Anatomical record  232  112-20  1992

Mostrar resumen
1536455 1536455
Antisera against the indolealkylamines: tryptophan, 5-hydroxytryptophan, 5-hydroxytryptamine, 5-methoxytryptophan, and 5-methoxytryptamine tested by an enzyme-linked immunosorbent assay method.
Geffard et al.
J. Neurochem., 44(4):1221-1228 (1985)  1985

3919158 3919158

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Categorías

Life Science Research > Antibodies and Assays > Primary Antibodies