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MABF920 Anti-SAP/SH2D1A Antibody, clone 1A9

Anti-SAP Antibody, clone 1A9 is an antibody against SH2D1A for use in Flow Cytometry, Western Blotting.

Anti-SAP/SH2D1A Antibody, clone 1A9 : Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Inflammation & Immunology Research Sub-Category: Immunological Signaling Gene Symbol: Sh2d1a, Xlp UniProt Number: O88890
MABF920
100 μg  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HFC, WBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF920
DescriptionAnti-SAP/SH2D1A Antibody, clone 1A9
Alternate Names
  • SH2 domain-containing protein 1A
  • Signaling lymphocytic activation molecule-associated protein
  • SLAM-associated protein
  • T-cell signal transduction molecule SAP
Background InformationSH2 domain-containing protein 1A (UniProt O60880; also known as Signaling lymphocytic activation molecule-associated protein, SLAM-associated protein, T-cell signal transduction molecule SAP) is encoded by the Sh2d1a (also known as Xlp) gene (Gene ID 20400) in murine species. SAP/SH2D1A is a 628-amino acid (isoform A) adaptor protein expressed in T cells, natural killer (NK) cells, NK-T cells, and some B cells. SAP/SH2D1A is made up almost exclusively of a Src homology 2 (SH2) domain (a.a. 6-104) and plays an important role in multiple immune cell functions, including T helper 2 (TH2) cytokine production, T cell-dependent antibody production, follicular T helper (TFH) cell differentiation, NK-T cell development, CD8+ T cell- and NK cell-mediated cytotoxicity. SAP couples signaling lymphocytic activation molecule (SLAM) family receptors to tyrosine phosphorylation signals through its interaction with the SH3 domain of Src family protein tyrosine kinase Fyn. In addition, SAP can prevent SH2 domain-containing negative regulators, such as phosphatases SHIP-1 and SHP-1, from inactivating SLAM-related receptors by blocking their interaction with the receptors. Evidence also shows that SAP/SH2D1A regulates T cell-dependent antibody production and TFH cell differentiation independent of Fyn. SAP/SH2D1A gene is mutated and inactivated in X-linked lymphoproliferative disease, a rare human immunodeficiency characterized by an uncontrolled immune response to Epstein-Barr virus infection.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-SAP Antibody, clone 1A9 is an antibody against SH2D1A for use in Flow Cytometry, Western Blotting.
Key Applications
  • Flow Cytometry
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected SAP in YT-S human NK cell lysates (Pérez-Quintero, L.A., et al. (2014). J. Exp. Med. 211(4):727-742).
Flow Cytometry Analysis: A representative lot immunostained wild-type, but not sh2d1a-knockout, murine peripheral blood CD3+CD4+ lymphocytes (Zhong, M.C., and Veillette, A. (2013). Mol. Cell. Biol. 33(6):1223-1232).
Western Blotting Analysis: A representative lot detected SAP, but not EAT-2 or ERT, exogenously expressed in the mouse mature mouse T cell line BI-141 (Roncagalli, R., et al. (2005). Nat. Immunol. 6(10):1002-1010).
Biological Information
ImmunogenGST-tagged recombinant full-length mouse SAP.
Clone1A9
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 1A9 reacts with SAP, but not EAT-2 or ERT (Roncagalli, R., et al. (2005). Nat. Immunol. 6(10):1002-1010). Clone 1A9 immunostained wild-type, but not sh2d1a-knockout murine peripheral blood CD3+CD4+ lymphocytes (Zhong, M.C., and Veillette, A. (2013). Mol. Cell. Biol. 33(6):1223-1232).
IsotypeIgG2aκ
Species Reactivity
  • Mouse
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Sh2d1a
  • Xlp
Purification MethodProtein G Purified
UniProt Number
Molecular Weight13.90/13.59 kDa (murine isoform 1/2) and 14.19 kDa (human isoform A) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 0.1 µg of this antibody detected SAP/SH2D1A in mouse splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABF920 04055977344257