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MABS1152 Anti-ROR2 Antibody, clone Nt 2535-2835

MABS1152
100 μg  
Purchase on Sigma-Aldrich

Ofertas especiales

Descripción

Replacement Information

Ofertas especiales

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWB, IHC, IH(P), ELISAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1152
DescriptionAnti-ROR2 Antibody, clone Nt 2535-2835
Alternate Names
  • Tyrosine-protein kinase transmembrane receptor ROR2
  • mRor2
  • Ntrkr2
  • Neurotrophic tyrosine kinase receptor-related 2
Background InformationTyrosine-protein kinase transmembrane receptor ROR2 (EC 2.7.10.1; UniProt Q9Z138; also known as mRor2, Ntrkr2, Neurotrophic tyrosine kinase receptor-related 2) is encoded by the Ror2 gene (Gene ID 26564) in murine species. Ror2 is a receptor tyrosine kinase that binds Wnt5a and negatively regulates canonical Wnt signaling. Although in C. elegans and Xenopus Ror2 is reported to modulate Wnt signaling nonautonomously by preventing Wnt from stimulating adjacent cells, studies conducted in mammalian cells suggest that Ror2 cytoplasmic domain is crucial for its function and Ror2 inhibits Wnt signaling in a cell-autonomous manner. Ror2 and the closely related Ror1 play essential roles during development. Human ROR2 gene mutations have been associated with dominant Brachydactyly B and recessive Robinow syndrome. Ror2-null mice exhibit skeletal abnormalities similar to the human syndromes. In addition, these mice display heart and lung defects and die perinatally. Ror2 is initially translated with an N-terminal signal sequence (a.a. 1-33), the removal of which yields the mature receptor with an extracellular region (a.a. 34-403), a transmembrane segment (a.a. 404-424), followed by the cytoplasmic region (a.a. 425-944) that contains the kinase domain (a.a. 473-746), a Pro-rich domain (a.a. 784-857) sandwiched between two Ser/Thr-rich domains (a.a. 753-782 and a.a. 859-882).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-ROR2 Antibody, clone Nt 2535-2835 is an antibody against ROR2 for use in Western Blotting, Immunohistochemistry, Immunohistochemistry (Paraffin), ELISA.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • ELISA
Application NotesImmunohistochemistry Analysis: A representative lot detected Ror2 immunoreactivity in parafffin-embedded leiomyosarcoma (LMS) tumor, lung cancer, and breast cancer tissue sections (Courtesy of Dr. Roel Nusse, Stanford University, CA).
Immunohistochemistry Analysis: A representative lot detected Ror2 expression in various regions of wild-type and Ror2+/-, but not Ror2-/-, mouse E13.5 embryos by both fluorescent and non-fluorescent immunohistochemistry using paraformaldehyde-fixed, paraffin-embedded whole mount sections (Mikels, A., et al. (2009). J. Biol. Chem. 284(44):30167-30176).
Immunohistochemistry Analysis: A representative lot detected Ror2 expression in the dermis of wild-type, but not Ror2-knockout mouse skin from E16.5 mouse embryos using paraformaldehyde-fixed, paraffin-embedded whole mount sections (van Amerongen, R., et al. (2012). Dev. Biol. 369(1):101-114).
Western Blotting Analysis: A representative lot detected full-length murine Ror2, as well as Ror2 deletion constructs lacking the first Ser/Thr-rich domain (ST1; a.a 753-782) or the Pro-rich domain (a.a. 784-857), but not Ror2 deletion mutants lacking the ST2 (a.a. 859-882) domain (Mikels, A., et al. (2009). J. Biol. Chem. 284(44):30167-30176).
Enzyme-linked Immunoabsorbent Assay (ELISA): The immunoreactivity of a representative lot toward the immunogen MBP fusion protein was confirmed by ELISA (Mikels, A., et al. (2009). J. Biol. Chem. 284(44):30167-30176).
Biological Information
ImmunogenRecombinant protein corresponding to the second Ser/Thr-rich domain of mouse mRor2.
EpitopeSecond Ser/Thr-rich domain.
CloneNt2535-2835
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone Nt 2535-2835 is specific to the mouse and human ROR2, but does not detect mouse ROR1. Clone Nt 2535-2835 exclusively detects ROR2 protein in paraffin-embedded tissues (Mikels, A., et al. (2009). J. Biol. Chem. 284(44):30167-30176).
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Ror2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~120 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MEF-1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ROR2 in 10 µg of MEF-1 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABS1152 04055977301236

Documentation

Anti-ROR2 Antibody, clone Nt 2535-2835 Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-ROR2 Antibody, clone Nt 2535-2835 Certificados de análisis

CargoNúmero de lote
Anti-ROR2, clone Nt 2535-2835 - 3246170 3246170
Anti-ROR2, clone Nt 2535-2835 - 3328859 3328859
Anti-ROR2, clone Nt 2535-2835 - 3476001 3476001
Anti-ROR2, clone Nt 2535-2835 - 3949170 3949170
Anti-ROR2, clone Nt 2535-2835 -Q2633066 Q2633066
Anti-ROR2, clone Nt 2535-2835 Monoclonal Antibody 3137643
Anti-ROR2, clone Nt 2535-2835 Monoclonal Antibody 2905268

Referencias bibliográficas

Visión general referenciasPub Med ID
Wnt5a can both activate and repress Wnt/β-catenin signaling during mouse embryonic development.
van Amerongen, R; Fuerer, C; Mizutani, M; Nusse, R
Developmental biology  369  101-14  2011

Mostrar resumen
22771246 22771246
Ror2 receptor requires tyrosine kinase activity to mediate Wnt5A signaling.
Mikels, A; Minami, Y; Nusse, R
The Journal of biological chemistry  284  30167-76  2009

Mostrar resumen
19720827 19720827