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MABS1235 Anti-Progesterone Receptor A/B Antibody, clone 488/H3

This Anti-Progesterone Receptor A/B Antibody, clone 488/H3 is validated for use in Western Blotting and Immunocytochemistry for the detection of Progesterone Receptor A/B.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: PGR, NR3C3 UniProt Number: P06401
MABS1235
100 μg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1235
DescriptionAnti-Progesterone Receptor A/B Antibody, clone 488/H3
Alternate Names
  • Progesterone receptor
  • PR
  • Nuclear receptor subfamily 3 group C member 3
  • Progesterone Receptor A/B
Background InformationProgesterone receptor (UniProt P06401; also known as nuclear receptor subfamily 3 group C member 3, PR) is encoded by the PGR (also known as NR3C3) gene (Gene ID 5241) in human. The progesterone receptor (PR) is a hormone activated transcription factor, where hormone binding triggers the dissociation of heat shock and chaperone proteins from PR, leading to PR dimerization and activation. The receptor dimer then binds progestin response elements in the regulatory regions of its target genes and a multi-component complex is assembled to enable transcription. In addition, a number of kinases are reported to regulate PR activity via phosphorylation. PR can also regulate transcription without direct DNA binding by tethering to other transcription factors, such as specificity protein 1 (Sp1) or activator protein 1 (AP-1), and modulating their transcriptional activity on target genes. PR is expressed as five isoforms, including PR-B and PR-A that are produced as a result of alternate estrogen inducible promoters within the same PGR gene. PR-A lacks the first 164 amino acids of PR-B, but the two isoforms have otherwise identical sequence. PR-B and PR-A can regulate distinct subsets of target genes and studies in knockout mice suggest that PR-B predominantly regulates mammary gland development and PR-A is critical for normal uterine function.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Progesterone Receptor A/B Antibody, clone 488/H3 is validated for use in Western Blotting and Immunocytochemistry for the detection of Progesterone Receptor A/B.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A representative lot detected exogenously expressed progesterone receptor isoform A and isoform B (PR-A & PR-B) in transfected MCF10A cells by fluorescent immunocytochemistry (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).
Western Blotting Analysis: A representative lot detected endogenous progesterone receptor isoforms A & B (PR-A & PR-B) in human breast cancer T47D cell lysate, as well as exogenously expressed PR-A & PR-B in transfected DCIS.COM human ductal carcinoma in situ (DCIS) breast cancer cells, but not in untransfected DCIS.COM cells (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).
Biological Information
ImmunogenRecombinant protein corresponding to the N-terminal half fragment of human Progesterone Receptor B.
EpitopeInternal
Clone488/H3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 488/H3 reacts with both isoform 1 (isoform B; PR-B) and isoform 2 (isoform A; PR-A). This clone is also expected to react with isoform 5 (delta4), but not isoform 3 or 4 (PR-M).
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PGR
  • NR3C3
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~110/80 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human breast cancer T47D cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected both isoforms A and B (PR-A & PR-B) of progesterone receptor in human breast cancer T47D cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABS1235 04055977288407

Documentation

Anti-Progesterone Receptor A/B Antibody, clone 488/H3 Ficha datos de seguridad (MSDS)

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Ficha técnica de seguridad del material (MSDS) 

Anti-Progesterone Receptor A/B Antibody, clone 488/H3 Certificados de análisis

CargoNúmero de lote
Anti-Progesterone Receptor A/B, -Q2607110 Q2607110
Anti-Progesterone Receptor A/B, clone 488/H3 - 3389934 3389934
Anti-Progesterone Receptor A/B, clone 488/H3 - 4030994 4030994

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