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AB5908-200UL Anti-Potassium Channel HERG Antibody, CT

Anti-Potassium Channel HERG Antibody, C-terminus is an antibody against Potassium Channel HERG for use in IC, IP & WB.

Anti-Potassium Channel HERG Antibody, CT: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: KCNH2, ERG1, LQT2, erg1, HERG, SQT1, H-ERG, Kv11.1, HERG1, Erg1, ERG UniProt Number: Q12809
AB5908-200UL
200 µL  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IP, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5908-200UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Potassium Channel HERG Antibody, CT
Alternate Names
  • Erg1
  • Kcnh2
References
Product Information
FormatAffinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of fusion protein (35 kD). The stock solution of the antigen can be made up using 100 μL of PBS. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. For positive control, use 20 ng of fusion protein per lane. Optimal concentrations must be determined by the end user.
PresentationAffinity Purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Quality LevelMQ100
Applications
ApplicationAnti-Potassium Channel HERG Antibody, C-terminus is an antibody against Potassium Channel HERG for use in IC, IP & WB.
Key Applications
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern blot: 1:200-400 using ECL on HERG transfected HEK293 cells.

Immunocytochemistry

Immunoprecipitation

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenGST fusion protein corresponding to amino acids 1106-1159 of human erg1 (HERG) (Accession number Q12809).
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes HERG (hKv11.1, Voltage gated K+ channel subfamily H member 2, KCNH2, ether-a-go-go related channel). The antibody recognizes the HERG1b splice variant but not splice variants HERG1-3 or HERG-4. The immunogen shares similiarity with rat erg2 (22/54) and rat erg3 (21/54).
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a voltage-activated potassium channel belonging to the eag family. It shares sequence similarity with the Drosophila ether-a-go-go (eag) gene. Mutations in this gene can cause long QT syndrome type 2 (LQT2). Transcript variants encoding distinct isoforms have been identified.
Gene Symbol
  • KCNH2
  • ERG1
  • LQT2
  • erg1
  • HERG
  • SQT1
  • H-ERG
  • Kv11.1
  • HERG1
  • Erg1
  • ERG
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q12809 # Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr). Isoform 3 has no channel activity by itself, but modulates channel characteristics when associated with isoform 1.
SIZE: 1159 amino acids; 126655 Da
SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH6/ERG2 and KCNH7/ERG3. Interacts with ALG10B (By similarity). Heteromultimer with KCNE1 and KCNE2.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in heart and brain.
DOMAIN:SwissProt: Q12809 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
PTM: Phosphorylated on serine and threonine residues. Phosphorylation by PKA inhibits ion conduction.
DISEASE: SwissProt: Q12809 # Defects in KCNH2 are the cause of long QT syndrome type 2 (LQT2) [MIM:152427]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. Deafness is often associated with LQT2. & Defects in KCNH2 are the cause of short QT syndrome type 1 (SQT1) [MIM:609620]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death.
SIMILARITY: Belongs to the potassium channel family. H (Eag) subfamily. & Contains 1 cyclic nucleotide-binding domain. & Contains 1 PAC (PAS-associated C-terminal) domain. & Contains 1 PAS (PER-ARNT-SIM) domain.
Molecular Weight127 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
AB5908-200UL 04053252278976

Documentation

Anti-Potassium Channel HERG Antibody, CT Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-Potassium Channel HERG Antibody, CT Certificados de análisis

CargoNúmero de lote
RABBIT ANTI-HERG (hKV11.1) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2110459 2110459
RABBIT ANTI-HERG (hKV11.1) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2384654 2384654
RABBIT ANTI-HERG (hKV11.1) - 2560993 2560993
RABBIT ANTI-HERG (hKV11.1) - 3896451 3896451
RABBIT ANTI-HERG (hKV11.1) - 4146482 4146482
RABBIT ANTI-HERG (hKV11.1) -2558353 2558353
RABBIT ANTI-HERG (hKV11.1) -2586510 2586510
RABBIT ANTI-HERG (hKV11.1) -2659193 2659193
RABBIT ANTI-HERG (hKV11.1) -2688777 2688777
RABBIT ANTI-HERG (hKV11.1) -2698403 2698403
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Ficha técnica

Cargo
RABBIT ANTI-HERG (hKV11.1) AFFINITY PURIFIED POLYCLONAL ANTIBODY