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05-719 Anti-Phosphatidylserine Antibody, clone 1H6

05-719
200 µg  
Purchase on Sigma-Aldrich

Ofertas especiales

Descripción

Ofertas especiales

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
VrtFC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue Number05-719
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Phosphatidylserine Antibody, clone 1H6
OverviewSignificance:
Anti-Phosphatidylserine may be used to detect translocation of the membrane phospholipid phosphatidylserine (PS) from the inner to the outer cell membrane leaflet; it provides an alternative to Annexin V for quantitation of apoptosis.
Background InformationPhosphatidylserine (PS) is a phospholipid (1,2-diacyl-sn-glycerol-(3)-L-phosphoserine) found in the membrane of all plants and animals. PS is most concentrated in the brain, as PS makes up about 10% of the lipid content of neurons. Neural PS is thought to play a role in maintaining nerve cell integrity, enhancing neurotransmitter signal efficiency, and improving nerve cell signal transmission. One of the key features of apoptosis is the expresion of PS on the outer cell membrane. PS is usually found on the inner side of the plasma membrane, but during early stage apoptosis, the PS is translocated to the outer membrane. FITC-labeled annexin V can bind to the exposed PS, providing the basis of most annexin V-based apoptosis assays.
Product Information
FormatPurified
Control
  • Brain tissue
PresentationPurified IgG supplied in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4 before the addition of glycerol to 30%. Liquid at -20°C.
Quality LevelMQ100
Applications
ApplicationAnti-Phosphatidylserine Antibody, clone 1H6 is an antibody against Phosphatidylserine for use in FC, IH.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
Application NotesFlow Cytometry: 0.2-2 μg/mL of a previous lot detected cell surface phosphatidylserine on Jurkat cells treated with Fas Ligand vesicles (Catalog # 01-210). See page two.

Immunohistochemistry: This antibody has been reported by an independent laboratory to detect phosphatidylserine in rat carotid artery paraffin sections fixed with ethanol/acetone (Mandinov, L., 2003).
Biological Information
ImmunogenLiposomes containing 70% phosphatidylserine and 30% phosphatidylglycerol
Cloneclone 1H6
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes phosphatidylserine (PS) in cell membranes
IsotypeIgG
Species Reactivity
  • Vertebrates
Antibody TypeMonoclonal Antibody
Modifications
  • Phosphorylation
Purification MethodProtein G Purified
Molecular Weightnone given
Product Usage Statements
Quality Assuranceroutinely evaluated by flow cytometry on Jurkat cells treated with Fas Ligand vesicles (Catalog #01-210)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at from date of receipt.
Packaging Information
Material Size200 µg
Global Trade Item Number
Número de referencia GTIN
05-719 04053252670220