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OP40 Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10)

This Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of Pan-Ras (Ab-3).

Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
OP40
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Descripción

Tabla espec. clave

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
OP40-100UG Ampolla de plást. 100 μg
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Description
OverviewRecognizes the ~21 kDa H-, K-, and N-Ras proteins in SW480 and Y1 cells and breast carcinoma tissue.
Catalogue NumberOP40
Brand Family Calbiochem®
Application Data
Detection of human Ras by immunoblotting: Sample: Whole cell lysate (56 μg) from MCF-7 cells. Primary antibody: Anti-Pan-Ras (Ab-3) Mouse mAb (RAS10) (Cat. No. OP40) (0.3 μg/ml). Detection: chemiluminescence.
References
ReferencesRodenhuis, S. et al., 1992. Cancer Res. (Suppl) 52, 2665s.
Sidransky, D., et al. 1992. Science 256, 102.
Hamer, P.J., et al. 1990. Hybridoma 9, 573.
Bos, J.L. 1989. Cancer Res. 49, 4682.
Furth, M. E., et al. 1987. Oncogene 1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Positive controlSW480 or Y1 cells or breast carcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone, Immunoblotting, Immunoprecipitation Hamer, P.J., et al. 1990. Hybridoma 9, 573.
Key Applications Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Paraffin Sections
Application NotesFlow Cytometry (2.5 µg/ml)
Frozen Sections (2.5 µg/ml)
Immunoblotting (0.1-3 µg/ml, chemiluminescent and colorimetric, see application references)
Immunofluorescence (2.5 µg/ml, see application references)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (2.5 µg/ml, saponin pre-treatment required)
Application CommentsThe level of expression of p21ras is variable in different tissues. For this reason we recommend a concentration step prior to immunoblot analysis to obtain optimal results. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant human p21 Ras
ImmunogenHuman
CloneRAS 10
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Número de referencia GTIN
OP40-100UG 07790788054458