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MABS197 Anti-PTP1B Antibody, clone FG6-1G

Anti-PTP1B Antibody, clone FG6-1G is an antibody against PTP1B for use in Western Blotting, Immunoprecipitation.

Anti-PTP1B Antibody, clone FG6-1G: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: PTPN1, PTP1B UniProt Number: P18031
MABS197
100 µg  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS197
Replaces04-1140
DescriptionAnti-PTP1B Antibody, clone FG6-1G
Alternate Names
  • Tyrosine-protein phosphatase non-receptor type 1
  • Protein-tyrosine phosphatase 1B
  • PTP-1B
Background InformationPTP1B is the prototypic member of the protein tyrosine phosphatase (PTP) family, which comprises at least 37 proteins. The family is characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. The PTPs have high substrate specificity for phosphotyrosyl proteins. At the primary sequence level, PTPs share little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP1B is a negative regulator of insulin and leptin signal transduction and may be a potential therapeutic target for diabetes and obesity. Insulin also influences the expression of splice variants of PTP1B.
References
Product Information
FormatPurified
Control
  • TF-1 cell lysate
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PTP1B Antibody, clone FG6-1G is an antibody against PTP1B for use in Western Blotting, Immunoprecipitation.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesWestern Blot Analysis: A representative lot was used to detect PTP1B in Jurkat cell lysate.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected PTP1B in A431 cell lysate (Gulati, P. et al. (2004). EMBO Rep. 5(8):812-817.).
Biological Information
ImmunogenRecombinant protein corresponding to the catalytic domain of human PTP1B.
EpitopeCatalytic domain
CloneFG6-1G
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes the catalytic domain of PTP1B.
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
Gene Symbol
  • PTPN1
  • PTP1B
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion.

CATALYTIC ACTIVITY: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

SUBUNIT STRUCTURE: Interacts with EPHA3 (phosphorylated); dephosphorylates EPHA3 and may regulate its trafficking and function.

SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note: Interacts with EPHA3 at the cell membrane.

POST-TRANSLATIONAL MODIFICATION: Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.

SEQUENCE SIMILARITIES: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. Contains 1 tyrosine-protein phosphatase domain.
Molecular Weight~50 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in TF-1 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PTP1B in 10 µg of TF-1 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABS197 04053252689826

Documentation

Anti-PTP1B Antibody, clone FG6-1G Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-PTP1B Antibody, clone FG6-1G Certificados de análisis

CargoNúmero de lote
Anti-PTP1B, clone FG6-1G 2479551
Anti-PTP1B, clone FG6-1G - 2386525 2386525
Anti-PTP1B, clone FG6-1G - 2432520 2432520
Anti-PTP1B, clone FG6-1G - 2376686 2376686
Anti-PTP1B, clone FG6-1G - 3167072 3167072
Anti-PTP1B, clone FG6-1G - 3387625 3387625
Anti-PTP1B, clone FG6-1G - 3585812 3585812
Anti-PTP1B, clone FG6-1G - 3720623 3720623
Anti-PTP1B, clone FG6-1G - 3901423 3901423
Anti-PTP1B, clone FG6-1G - 4080485 4080485
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Referencias bibliográficas

Visión general referenciasPub Med ID
UVA inactivates protein tyrosine phosphatases by calpain-mediated degradation.
Gulati, Pawan, et al.
EMBO Rep., 5: 812-7 (2004)  2004

Mostrar resumen 		15247926 15247926

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