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ST1202 Anti-PGC-1α Mouse mAb (4C1.3)

This Anti-PGC-1α Mouse mAb (4C1.3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation, Paraffin Sections for the detection of PGC-1α.

Anti-PGC-1α Mouse mAb (4C1.3): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: Anti-Peroxisome Proliferator-Activated Receptor- γ Coactivator 1 α

ST1202
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
ST1202-1SET Ampolla de plást. 1 set
Description
OverviewRecognizes the endogenous forms of PGC-1α, which includes the ~113 kDa PGC-1α protein and the ~38 kDa splice variant, in brown adipose tissue, liver, and kidney.
Catalogue NumberST1202
Brand Family Calbiochem®
SynonymsAnti-Peroxisome Proliferator-Activated Receptor- γ Coactivator 1 α
Application Data
Detection of human PGC-1α by immunoblottiong. Samples: Whole cell lysates from COS cells transformed with wild-type PGC-1α (5 µg, lane 1; 10 µg, lane 2; 20 µg, lane 3), whole cell extract from brown adipose tissue of a cold-exposed mouse (lane 4), and whole cell lysate from CHO cells transformed with NT-PGC-1α (0.5 µg, lane 5). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection chemiluminescence.

Detection of mouse PGC-1α by immunoblottiong. Samples: NT-PGC-1α Positive Control, (lane 1), whole cell extract from brown adipose tissue of mice kept at room temperature (50 µg; lanes 2-4), whole cell extract from brown adipose tissue of mice exposed to 4°C for 5 h (50 µg; lanes 5-7), and PGC-1α positive control (lane 8). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection: chemiluminescence.

Detection of mouse PGC-1α by immunoblottiong. Samples: PGC-1α positive control (lane 1), nuclear extract from mouse liver (75 µg, lane 2), whole cell extract from mouse kidney (100 µg, lane 3), and whole cell extract from kidney of a PGC-1α knock-out mouse (100 µg, lane 4). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesLai, L., et al. 2008. Genes Dev. 14, 1948.
Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
Product Information
FormLiquid
FormulationOne vial of 100 µg antibody in 50 mM PBS, see vial for lot-specific concentration. One vial of NT-PGC-1α Positive Control, supplied as 25 µl whole cell extract in RIPA buffer containing 10 mM &beta-mercaptoethanol and 1% SDS; load 10 µl per lane; add sample buffer prior to SDS-PAGE loading.
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesParaffin Sections
Koh, Y.J., et al. 2009. Exp. Mol. Med. 41, 880.
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (1 µg/ml)
Immunocytochemistry (5-10 µg/ml)
Immunoprecipitation (10 µg/ml)
Paraffin Sections (whole mount) (see application references)
Application CommentsEndogenous PGC-1α is found primarily in the nucleus and NT-PGC-1α is found in both cytosolic and nuclear fractions. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of amino acids 1-120 of mouse PGC-1α
ImmunogenMouse
Clone4C1.3
HostMouse
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 21/22-36/37/38

Harmful in contact with skin and if swallowed.
Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36/37-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing and gloves.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
ST1202-1SET 04055977224085

Documentation

Anti-PGC-1α Mouse mAb (4C1.3) Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-PGC-1α Mouse mAb (4C1.3) Certificados de análisis

CargoNúmero de lote
ST1202

Referencias bibliográficas

Visión general referencias
Lai, L., et al. 2008. Genes Dev. 14, 1948.
Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.

Citas

Título
  • Chang, J.S., et al. 2010. J. Biol. Chem. In Press.
  • Estall, J. L., et al. 2009. Diabetes 58, 1499.
  • Zhang Y., et al. 2009. J. Biol. Chem.) in Press.

  • Paraffin Sections
    Koh, Y.J., et al. 2009. Exp. Mol. Med. 41, 880.
    		•
    Ficha técnica

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision23-October-2018 JSW
    SynonymsAnti-Peroxisome Proliferator-Activated Receptor- γ Coactivator 1 α
    ApplicationImmunoblotting (1 µg/ml)
    Immunocytochemistry (5-10 µg/ml)
    Immunoprecipitation (10 µg/ml)
    Paraffin Sections (whole mount) (see application references)
    Application Data
    Detection of human PGC-1α by immunoblottiong. Samples: Whole cell lysates from COS cells transformed with wild-type PGC-1α (5 µg, lane 1; 10 µg, lane 2; 20 µg, lane 3), whole cell extract from brown adipose tissue of a cold-exposed mouse (lane 4), and whole cell lysate from CHO cells transformed with NT-PGC-1α (0.5 µg, lane 5). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection chemiluminescence.

    Detection of mouse PGC-1α by immunoblottiong. Samples: NT-PGC-1α Positive Control, (lane 1), whole cell extract from brown adipose tissue of mice kept at room temperature (50 µg; lanes 2-4), whole cell extract from brown adipose tissue of mice exposed to 4°C for 5 h (50 µg; lanes 5-7), and PGC-1α positive control (lane 8). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection: chemiluminescence.

    Detection of mouse PGC-1α by immunoblottiong. Samples: PGC-1α positive control (lane 1), nuclear extract from mouse liver (75 µg, lane 2), whole cell extract from mouse kidney (100 µg, lane 3), and whole cell extract from kidney of a PGC-1α knock-out mouse (100 µg, lane 4). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection: chemiluminescence.
    DescriptionProtein G purified mouse monclonal antibody. Recognizes the endogenous forms of PGC-1α, which includes the ~113 kDa PGC-1α protein and the ~38 kDa splice variant.
    BackgroundPGC-1α is a co-activator of PPARα, PPARγ, and other transcription factors and regulates the transcriptonal program of adaptive thermogenesis in brown adipose tissue, hepatic/renal gluconeogenesis, and muscle fiber type switching. The full-length protein is 113 kDa and is induced in brown adipose tissue by cold exposure, in liver and kidney by fasting, and in skeletal muscle by exercise. Alternative splicing of the full-length gene produces a 270 aa N-terminal splice variant that migrates at ~38 kDa and is induced by the same physiological signals that induce expression of the full-length protein. The N-terminal PGC-1α splice variant is also subject to post translational modifications that alter its migration and apparent molecular weight.
    HostMouse
    Immunogen speciesMouse
    Immunogena recombinant protein consisting of amino acids 1-120 of mouse PGC-1α
    Clone4C1.3
    IsotypeIgG
    Specieshuman, mouse, rat
    FormLiquid
    FormulationOne vial of 100 µg antibody in 50 mM PBS, see vial for lot-specific concentration. One vial of NT-PGC-1α Positive Control, supplied as 25 µl whole cell extract in RIPA buffer containing 10 mM &beta-mercaptoethanol and 1% SDS; load 10 µl per lane; add sample buffer prior to SDS-PAGE loading.
    PreservativeNone
    CommentsEndogenous PGC-1α is found primarily in the nucleus and NT-PGC-1α is found in both cytosolic and nuclear fractions. Antibody should be titrated for optimal results in individual systems.
    Storage Avoid freeze/thaw
    ≤ -70°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
    Toxicity Multiple Toxicity Values, refer to MSDS
    ReferencesLai, L., et al. 2008. Genes Dev. 14, 1948.
    Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
    Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
    Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
    Citation
  • Chang, J.S., et al. 2010. J. Biol. Chem. In Press.
  • Estall, J. L., et al. 2009. Diabetes 58, 1499.
  • Zhang Y., et al. 2009. J. Biol. Chem.) in Press.

  • Paraffin Sections
    Koh, Y.J., et al. 2009. Exp. Mol. Med. 41, 880.
    		•
    Application referencesParaffin Sections
    Koh, Y.J., et al. 2009. Exp. Mol. Med. 41, 880.