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ABC1691 Anti-PEPCK

Detect Phosphoenolpyruvate carboxykinase, cytosolic [GTP] using this rabbit polyclonal Anti-PEPCK, Cat. No. ABC1691, tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-PEPCK: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Apoptosis & Cancer Gene Symbol: PCK1, PEPCK1 UniProt Number: P35558
ABC1691
100 μg  
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC1691
DescriptionAnti-PEPCK
Alternate Names
  • Phosphoenolpyruvate carboxykinase
  • cytosolic
  • PEPCK-C
Background InformationPhosphoenolpyruvate carboxykinase, cytosolic (UniProt: also known as PEPCK, PEPCK-C) is encoded by the PCK1 (also known as PEPCK1) gene (Gene ID: 5105) in human. PEPCK catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), which is a rate-limiting step in gluconeogenesis. It requires manganese as a cofactor. It is expressed in higher levels in liver of fasting subjects. Kidney and adipocytes exhibit lower expression levels. Two isozymes of PEPCK have been identified, cytosolic and mitochondrial. PEPCK consists of an N-terminal and a catalytic C-terminal domain, with the active site and metal ions located in a cleft between them. Upon binding to substrate, PEPCK undergoes a conformational change that accelerates the catalysis process. Expression of PEPCK can be controlled by cAMP, glucocorticoids, and by insulin. Its expression is induced by glucagon, catecholamines, and glucocorticoids during periods of fasting and in response to stress and is diminished following glucose-induced and by the action of insulin. PEPCK-C is absent in fetal liver but appears at birth, concomitant with the capacity for gluconeogenesis. Deficiency of PEPCK-C is a rare metabolic disorder results in fatty infiltration of liver and kidney, hypoglycemia, hepatomegaly, and failure to thrive.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Phosphoenolpyruvate carboxykinase, cytosolic [GTP] using this rabbit polyclonal Anti-PEPCK, Cat. No. ABC1691, tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PEPCK in human kidney, human pancreas, human skeletal muscle, and human placental tissue.

Western Blotting Analysis: 2 µg/mL from a representative lot detected PEPCK in 10 µg of human kidney tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal half of human phosphoenolpyyruvate carboxykinase (PEPCK).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects cytosolic phosphoenolpyruvate carboxykinase in human kidney.
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PCK1
  • PEPCK1
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~65 kDa observed; 69.19 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human liver tissue

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected PEPCK in human liver tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
ABC1691 04054839189128