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MABT126 Anti-Obscurin Antibody, clone 5H10A

This mouse monoclonal Anti-Obscurin, clone 5H10A Antibody, Cat. No. MABT126 is tested in Immunofluorescence Immunohistochemistry, and Western Blotting for the detection of obscurin.

Anti-Obscurin Antibody, clone 5H10A: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Cell Structure Gene Symbol: OBSCN, KIAA1556, KIAA1639 UniProt Number: Q5VST9
MABT126
200 µL  
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Descripción

Replacement Information

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IHC, IFMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT126
DescriptionAnti-Obscurin Antibody, clone 5H10A
Alternate Names
  • EC:2.7.11.1
  • Obscurin-RhoGEF
  • Obscurin-myosin light chain kinase
  • Obscurin-MLCK
Background InformationObscurin (UniProt: Q5VST9; also known as EC:2.7.11.1, Obscurin-RhoGEF, Obscurin-myosin light chain kinase, Obscurin-MLCK) is encoded by the OBSCN (also known as KIAA1556, KIAA1639) gene (Gene ID: 84033) in human. Obscurin is a member of the family of giant sarcomeric signaling proteins that includes titin and nebulin. It is expressed in cardiac and skeletal muscle, and plays a role in the organization of myofibrils during sarcomere assembly. Obscurin contains 68 Ig domains, 2 fibronectin domains, 1 calcium/calmodulin-binding domain, 1 RhoGEF domain with an associated PH domain, and 2 serine-threonine kinase domains. The dominant location of obscurin in mature myofibrils is shown to be at the peripheries of Z-disks and M-lines, where its position allows it to communicate with the surrounding myoplasm. Obscurin is reported to associates with the contractile apparatus through its binding to titin, sarcomeric myosin, and perhaps other proteins of the contractile apparatus. Obscurin is tightly associated with the mature contractile apparatus, hence it displays only limited solubility under non-denaturing conditions. However, it can be extracted from adult muscle fibers only in the presence of 2% SDS and 1% beta-mercaptoethanol. It is shown that primary cultures of neonatal skeletal myotubes contain large amounts of the 800 kDa obscurin isoform that can be solubilized with neutral detergents during early stages of sarcomere formation. (Ref.: Kontrogianni-Konstantopoulos, A., and Bloch, RJ. (2005). J Muscle Res Cell Motil. 26(6-8):419-26).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal Anti-Obscurin, clone 5H10A Antibody, Cat. No. MABT126 is tested in Immunofluorescence Immunohistochemistry, and Western Blotting for the detection of obscurin.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunofluorescence
Application NotesWestern Blotting Analysis: A representative lot detected Obscurin in Western Blotting applications (Bowman, A.L., et. al. (2007). FEBS Lett. 581(8):1549-54; Ackermann, M.A., et. al. (2014). PLoS One. 9(2):e88162).

Immunofluorescence Analysis: A representative lot detected Obscurin in Immunofluorescence applications (Bowman, A.L., et. al. (2007). FEBS Lett. 581(8):1549-54).

Immunofluorescence Analysis: A representative lot detected Obscurin in mouse heart left ventricle and mouse quadriceps muscle (Courtesy of Li-Yen R. Hu, Ph.D., and Aiketerini Kontrogianni-Konstantopoulos, Ph.D. , University of Maryland, School of Medicine, USA).

Immunohistochemistry Analysis: A representative lot detected Obscurin in Immunohistochemistry applications (Kontrogianni-Konstantopoulos, A., et. al. (2006). FASEB J. 20(12):2102-11; Ackermann, M.A., et. al. (2014). PLoS One. 9(2):e88162).

Western Blotting Analysis: A representative lot detected Obscurin in mouse heart left ventricle and mouse tibialis anterior muscle tissues (Courtesy of Li-Yen R. Hu, Ph.D., and Aiketerini Kontrogianni-Konstantopoulos, Ph.D. , University of Maryland, School of Medicine, USA).
Biological Information
ImmunogenGST-tagged full-length human recombinant Obscurin.
EpitopeN-terminus
Clone5H10A
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5H10A detects obscurin in heart and skeletal muscle.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • OBSCN
  • KIAA1556
  • KIAA1639
Purification MethodProtein G purified
UniProt Number
Molecular Weight~800 kDa observed; 868.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABT126 04054839193552