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MABN737 Anti-OPA1, clone 1OPA-1A8 Antibody

MABN737
100 µL  
Purchase on Sigma-Aldrich

Ofertas especiales

Descripción

Ofertas especiales

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICC, IPMAscitesMonoclonal Antibody
Description
Catalogue NumberMABN737
DescriptionAnti-OPA1, clone 1OPA-1A8 Antibody
Alternate Names
  • Dynamin-like 120 kDa protein, mitochondrial
  • Large GTP-binding protein
  • LargeG
  • Optic atrophy protein 1 homolog
  • Dynamin-like 120 kDa protein, form S1
Background InformationOPA1 (optic atrophy 1) is a dynamin-related GTPase that plays a role in genome preservation and mitochondrial morphology. Localization of OPA1 is thought to be anchored to the inner membrane of the mitochondria. OPA1 is highly expressed in inner and outer retinal neurons and is important for normal mitochondrial function in those cells. Mutations in OPA1 are the major cause of autosomal dominant optic atrophy. Recent research has found that OPA1 also has the ability to protect neurons from excitotoxic injury and could be a potential clinical target to increase endurance of neurons post brain damage.
Product Information
FormatAscites
PresentationMouse monoclonal IgG1κ ascites with no preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-OPA1 antibody, clone 1OPA-1A8 is validated for use in western blotting, ICC & IP for the detection of OPA1.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected OPA1 in COS cells (Prof. Mustapha Oulad-Abdelghani, IGBMC, France).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated OPA1 from mouse brain tissue lysate (Akepati, V. R., et al. (2008). J Neurochem. 106(1):372-383.).
Biological Information
ImmunogenRecombinant protein corresponding to mouse OPA1.
Clone10PA-1A8
HostMouse
SpecificityThis antibody detects six known isoforms of OPA1 (Akepati, V. R., et al. (2008). J Neurochem. 106(1):372-383.).
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Opa1
Purification MethodUnpurified
UniProt Number
Molecular Weight~90 and ~100 kDa observed. Six isoforms between 80 kDa and 100 kDa are known to exist (Akepati, V. R., et al. (2008). J Neurochem. 106(1):372-383.).
Product Usage Statements
Quality AssuranceEvaluated by human brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected OPA1 in 10 µg of human brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Global Trade Item Number
Número de referencia GTIN
MABN737 04053252944802