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AB5411 Anti-Nitrotyrosine Antibody

AB5411
100 µg  
Purchase on Sigma-Aldrich

Ofertas especiales

Descripción

Replacement Information

Ofertas especiales

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
AIH(P), WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberAB5411
ReplacesAB5386
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Nitrotyrosine Antibody
Background InformationThe cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • POSITIVE CONTROL: Tyrosine residues can be nitrated directly in situ on tissue sections using peroxynitrite (catalog # 20-107) to create a positive control. After the tissue section is deparaffinized, the slide is covered with a drop of PBS, pH 7.4 into which 10 microliters of peroxynitrite solution are mixed. Care should be taken that the solution maintains a pH of 7.4.
PresentationPurified rabbit polyclonal in buffer containing 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Nitrotyrosine using this Anti-Nitrotyrosine Antibody validated for use in IH(P) & WB.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesWestern Blot:
1:100-1:1,000 dilution of a previous lot was used sucessfully in western blot when tested against nitrated BSA and nitrated ovalbumin.

Immunohistochemistry:
1:50-1:100 on paraffin embedded, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Incubation with the AB5411 was for 10 minutes and the reaction was developed with the Chemicon IHCSelect, HRP-DAB kit (Catalog number DAB150).

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenNitrated KLH.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes nitrated tyrosine residues.
Species Reactivity
  • All
Species Reactivity NoteHuman and mouse. Reactivity with other species has not been determined.
Antibody TypePolyclonal Antibody
Purification MethodProtein A Purfied
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceImmunohistochemistry(paraffin) Analysis:
Nitrotyrosine (AB5411) staining of Mouse Brain (Zymosan), tissue pretreated with citrate buffer, pH 6.0. Polyclonal antibody was diluted to 1:500, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen in here in hippocampus.
Optimal Staining of Nitrotyrosine Monoclonal: Mouse Brain Treated With Zymosan.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
AB5411 04053252284434

Documentation

Protocols

Title
AB5411 IH(P) Summary
IHC Select Paraffin Protocol

Anti-Nitrotyrosine Antibody Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-Nitrotyrosine Antibody Certificados de análisis

CargoNúmero de lote
Anti-Nitrotyrosine - 2142175 2142175
Anti-Nitrotyrosine - 2373395 2373395
Anti-Nitrotyrosine - 2436421 2436421
Anti-Nitrotyrosine - 2042778 2042778
Anti-Nitrotyrosine - 2291670 2291670
Anti-Nitrotyrosine - 3207617 3207617
Anti-Nitrotyrosine - 3244053 3244053
Anti-Nitrotyrosine - 3474160 3474160
Anti-Nitrotyrosine - 3597278 3597278
Anti-Nitrotyrosine - 3734509 3734509

Referencias bibliográficas

Visión general referenciasAplicación Pub Med ID
Pleiotropic Effects of Levofloxacin, Fluoroquinolone Antibiotics, against Influenza Virus-Induced Lung Injury.
Enoki, Y; Ishima, Y; Tanaka, R; Sato, K; Kimachi, K; Shirai, T; Watanabe, H; Chuang, VT; Fujiwara, Y; Takeya, M; Otagiri, M; Maruyama, T
PloS one  10  e0130248  2015

Mostrar resumen
26086073 26086073
Exposure to 100% Oxygen Abolishes the Impairment of Fracture Healing after Thoracic Trauma.
Kemmler, J; Bindl, R; McCook, O; Wagner, F; Gröger, M; Wagner, K; Scheuerle, A; Radermacher, P; Ignatius, A
PloS one  10  e0131194  2015

Mostrar resumen
26147725 26147725
Ferrous sulfate, but not iron polymaltose complex, aggravates local and systemic inflammation and oxidative stress in dextran sodium sulfate-induced colitis in rats.
Toblli, JE; Cao, G; Angerosa, M
Drug design, development and therapy  9  2585-97  2015

Mostrar resumen
26005335 26005335
Overexpression of TFAM or twinkle increases mtDNA copy number and facilitates cardioprotection associated with limited mitochondrial oxidative stress.
Ikeda, M; Ide, T; Fujino, T; Arai, S; Saku, K; Kakino, T; Tyynismaa, H; Yamasaki, T; Yamada, K; Kang, D; Suomalainen, A; Sunagawa, K
PloS one  10  e0119687  2015

Mostrar resumen
25822152 25822152
Ferric carboxymaltose-mediated attenuation of Doxorubicin-induced cardiotoxicity in an iron deficiency rat model.
Toblli, JE; Rivas, C; Cao, G; Giani, JF; Funk, F; Mizzen, L; Dominici, FP
Chemotherapy research and practice  2014  570241  2014

Mostrar resumen
24876963 24876963
Autophagy defends pancreatic β cells from human islet amyloid polypeptide-induced toxicity.
Rivera, JF; Costes, S; Gurlo, T; Glabe, CG; Butler, PC
The Journal of clinical investigation  124  3489-500  2014

Mostrar resumen
25036708 25036708
Therapeutic impact of human serum albumin-thioredoxin fusion protein on influenza virus-induced lung injury mice.
Tanaka, R; Ishima, Y; Enoki, Y; Kimachi, K; Shirai, T; Watanabe, H; Chuang, VT; Maruyama, T; Otagiri, M
Frontiers in immunology  5  561  2014

Mostrar resumen
25414704 25414704
Decreasing mitochondrial fission alleviates hepatic steatosis in a murine model of nonalcoholic fatty liver disease.
Galloway, CA; Lee, H; Brookes, PS; Yoon, Y
American journal of physiology. Gastrointestinal and liver physiology  307  G632-41  2014

Mostrar resumen
25080922 25080922
Long-acting human serum albumin-thioredoxin fusion protein suppresses bleomycin-induced pulmonary fibrosis progression.
Tanaka, R; Watanabe, H; Kodama, A; Chuang, VT; Ishima, Y; Hamasaki, K; Tanaka, K; Mizushima, T; Otagiri, M; Maruyama, T
The Journal of pharmacology and experimental therapeutics  345  271-83  2013

Mostrar resumen
23442250 23442250
The anti-oxidative, anti-inflammatory, and protective effect of S100A8 in endotoxemic mice.
Sun, Y; Lu, Y; Engeland, CG; Gordon, SC; Sroussi, HY
Molecular immunology  53  443-9  2013

Mostrar resumen
23127860 23127860

Folleto

Cargo
Pathways and Biomarkers of Oxidative Stress

Licencias necesarias e Información técnica

Cargo
White Paper- Modern Methods in Oxidative Stress Research

Ficha técnica

Cargo
Anti-Nitrotyrosine - Data Sheet