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PC223L Anti-Neuropeptide Y Rabbit pAb

This Anti-Neuropeptide Y Rabbit pAb is validated for use in Frozen Sections for the detection of Neuropeptide Y.

Anti-Neuropeptide Y Rabbit pAb: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
PC223L
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
Am, Av, Fe, F, H, Newt, RRbPolyclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
PC223L-100UL Ampolla de plást. 100 ul
Description
OverviewRecognizes neuropeptide Y in cortex, caudate, putamen, and paraventricular nucleus.
Catalogue NumberPC223L
Brand Family Calbiochem®
Application Data
Detection of rat neuropeptide Y by immunohistochemistry. Sample: Rat paraventricular nucleus, cortex, and caudate putamen. Primary antibody: Anti-Neuropeptide Y Rabbit pAb (Cat. No. PC223L) (1:10,000). Detection: fluorescence.
References
ReferencesSudhof, T.C. 1995. Nature 375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
Product Information
FormLyophilized
FormulationUndiluted serum.
Positive controlParaventricular nucleus, cortex, or caudate putamen
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Application NotesFrozen Sections (1:1000-1:2000 for free-floating, PAP detection)
Application CommentsAntibody specificity was examined in the rat cortex, caudate putamen, and paraventricular nucleus. Staining is completely eliminated by pretreatment of antibody with neuropeptide Y (10-6M). Preabsorption with avian pancreatic polypeptide, β-endorphin, cholecystokinin, peptide YY, somatostatin, or vasoactive intestinal peptide resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenporcine neuropeptide Y
ImmunogenPorcine
HostRabbit
IsotypeIgG
Species Reactivity
  • Amphibian
  • Avian
  • Feline
  • Fish
  • Human
  • Newt
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
PC223L-100UL 04055977224672

Documentation

Anti-Neuropeptide Y Rabbit pAb Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-Neuropeptide Y Rabbit pAb Certificados de análisis

CargoNúmero de lote
PC223L

Referencias bibliográficas

Visión general referencias
Sudhof, T.C. 1995. Nature 375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-October-2007 RFH
ApplicationFrozen Sections (1:1000-1:2000 for free-floating, PAP detection)
Application Data
Detection of rat neuropeptide Y by immunohistochemistry. Sample: Rat paraventricular nucleus, cortex, and caudate putamen. Primary antibody: Anti-Neuropeptide Y Rabbit pAb (Cat. No. PC223L) (1:10,000). Detection: fluorescence.
DescriptionRabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes neuropeptide Y.
BackgroundNeurotransmitters can be defined as chemical substances that are released by the presynaptic neuron before being bound by receptors on the postsynaptic cell surface. It has been well established that the nervous system utilizes neurotransmitters for cell signaling. Neurotransmitters can be divided into two groups; low molecular weight transmitter molecules such as biogenic amines, purines, or amino acids, and bioactive peptides, of which more than 50 members have been identified in the mammalian central nervous system. Many low molecular weight neurotransmitters are produced within the axon terminal from common cellular metabolites and packaged into synaptic vesicles. In contrast, neuropeptides are synthesized in the cell body and packaged into large dense core vesicles for delivery to the presynaptic membrane. The transport and delivery of vesicles containing neurotransmitter is a highly regulated and complex process. Neuropeptide Y (NPY) is a 36 amino acid peptide belonging to the pancreatic polypeptide family of neuroendocrine hormones. NPY is abundantly expressed in neurons within the central and peripheral nervous systems, including the hypothalamus and pituitary. NPY is a potent stimulator of food intake, and may also influence other growth hormones. A family of neuropeptide Y receptors (Y1-Y5) have been identified which recognize both neuropeptide Y and the related Peptide YY. The immunolocalization, neuronal distribution, and vesicular compartmentalization of neurotransmitter molecules and their precursors are important for understanding the modulation, regulation, and function of neurotransmitters in the nervous system.
HostRabbit
Immunogen speciesPorcine
Immunogenporcine neuropeptide Y
IsotypeIgG
Speciesamphibian, avian, feline, fish, human, newt, rat
Positive controlParaventricular nucleus, cortex, or caudate putamen
FormLyophilized
FormulationUndiluted serum.
Preservative≤0.1% sodium azide
CommentsAntibody specificity was examined in the rat cortex, caudate putamen, and paraventricular nucleus. Staining is completely eliminated by pretreatment of antibody with neuropeptide Y (10-6M). Preabsorption with avian pancreatic polypeptide, β-endorphin, cholecystokinin, peptide YY, somatostatin, or vasoactive intestinal peptide resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesSudhof, T.C. 1995. Nature 375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.