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ABE490 Anti-NSD2 Antibody

Detect NSD using this rabbit polyclonal antibody, Anti-NSD2 Antibody validated for use in western blotting, IHC & IP.

Anti-NSD2 Antibody: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: WHSC1, MMSET, NSD2, TRX5 UniProt Number: O96028
ABE490
100 µg  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE490
DescriptionAnti-NSD2 Antibody
Alternate Names
  • Nuclear SET domain-containing protein 2
  • NSD2
  • Histone-lysine N-methyltransferase NSD2
  • Multiple myeloma SET domain-containing protein
  • MMSET
  • Protein trithorax-5 and Wolf-Hirschhorn syndrome candidate 1 protein
  • WHSC1
Background InformationNuclear SET domain-containing protein 2 (NSD2) is also called Histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein (MMSET), Protein trithorax-5 and Wolf-Hirschhorn syndrome candidate 1 protein (WHSC1). NSD2 is a histone methyltransferase that acts on histone H3 'Lys-27' (H3K27me). Isoform RE-IIBP is thought to be a transcription regulator that uses HADC recruitment to binding DNA and suppress IL5 transcription. Chromosomal aberrations to WHSC1 are linked to multiple myeloma tumors.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect NSD using this rabbit polyclonal antibody, Anti-NSD2 Antibody validated for use in western blotting, IHC & IP.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunoprecipitation
Application NotesImmunohistochemistry Analysis: A 1:500 dilution from a representative lot detected NSD2 in human cerebellum and thalamus tissues.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected NSD2 in human kidney tissues.

Immunoprecipitation Analysis: 5 µg of this antibody immunoprecipitated NSD2 from 500 µg of HCT-116 cell lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a region near the C-terminus of human NSD2.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody is expected to cross-react with isoforms 1 (152 kDa), 3 (71 kDa), and 4 (66 kDa, REII-BP; IL-5 promoter REII-region-binding protein) based upon immunogen design.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • WHSC1
  • MMSET
  • NSD2
  • TRX5
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~150 kDa observed. Uniprot describes 7 different isoforms ranging from 30 kDa to 152 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected NSD2 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
ABE490 04053252926068