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ST1699 Anti-NDE1 Mouse mAb (2G11-1C11)

This Anti-NDE1 Mouse mAb (2G11-1C11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of NDE1.

Anti-NDE1 Mouse mAb (2G11-1C11): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: Anti-nudE, Anti-Nuclear Distribution Gene E Homolog1

ST1699
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
ST1699-100UG 100 μg
Description
OverviewRecognizes the ~40-45 kDa NDE1 protein in HeLa cells.
Catalogue NumberST1699
Brand Family Calbiochem®
SynonymsAnti-nudE, Anti-Nuclear Distribution Gene E Homolog1
Application Data
Detection of human NDE1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-NDE1 Mouse mAb (2G11-1C11) (Cat. No. ST1699) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human NDE1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-NDE1 Mouse mAb (2G11-1C11) (Cat. No. ST1699) (10 µg/ml). Detection: fluorescence.
References
ReferencesBradshaw, N.J., et al. 2009. Neurosci. Lett. 449, 228.
Product Information
Formliquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see coments)
Immunoblotting (1-4 µg/ml)
Immunocytochemistry (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human NDE1 (aa 1-336), expressed as a GST fusion protein
Clone2G11-1C11
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
ST1699-100UG 04055977207316

Documentation

Anti-NDE1 Mouse mAb (2G11-1C11) Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Referencias bibliográficas

Visión general referencias
Bradshaw, N.J., et al. 2009. Neurosci. Lett. 449, 228.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-November-2010 RFH
SynonymsAnti-nudE, Anti-Nuclear Distribution Gene E Homolog1
ApplicationELISA (see coments)
Immunoblotting (1-4 µg/ml)
Immunocytochemistry (10 µg/ml)
Application Data
Detection of human NDE1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-NDE1 Mouse mAb (2G11-1C11) (Cat. No. ST1699) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human NDE1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-NDE1 Mouse mAb (2G11-1C11) (Cat. No. ST1699) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~40-45 kDa NDE1 protein.
BackgroundThis gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Alternative splicing results in multiple transcript variants.
HostMouse
ImmunogenFull-length, recombinant, human NDE1 (aa 1-336), expressed as a GST fusion protein
Clone2G11-1C11
IsotypeIgG₁
Specieshuman
Positive controlHeLa cells
Formliquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesBradshaw, N.J., et al. 2009. Neurosci. Lett. 449, 228.