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MABE132 Anti-MyoD Antibody, clone 5F11

Detect MyoD using this Anti-MyoD Antibody, clone 5F11 validated for use in Western Blotting, ICC.

Anti-MyoD Antibody, clone 5F11: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: Myod1, Myod UniProt Number: P10085
MABE132
100 µg  
Purchase on Sigma-Aldrich

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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWB, ICCRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE132
ReplacesMABE514
DescriptionAnti-MyoD Antibody, clone 5F11
Alternate Names
  • Myoblast determination protein 1
Background InformationMyoblast determination protein 1 (MyoD1) is a myogenic transcription factor required for the growth, repair, and regeneration of muscle cells. It induces differentiation of myoblast cells into multinucleated myotubules, activating genes and proteins involved in cell cycle arrest, and differentiation. MyoD1 may also control proliferation and homeostasis during embryogenesis, in the absence of Myf5.
References
Product Information
FormatPurified
Control
  • C2C12 cell lysate
PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect MyoD using this Anti-MyoD Antibody, clone 5F11 validated for use in Western Blotting, ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A representative lot detected MyoD in C2C12 cells.

Western Blot Analysis: A representative lot detected MyoD in C2C12 cells.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to mouse MyoD.
Clone5F11
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Myod1
  • Myod
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~45 kDa observed. An uncharacterized band at ~38 kDa may be observed in some cell lysates. The calculated molecular weight is 34 kDa; however, this protein is known to be glycosylated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in C2C12 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected MyoD in 10 µg of C2C12 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABE132 04053252440830

Documentation

Anti-MyoD Antibody, clone 5F11 Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-MyoD Antibody, clone 5F11 Certificados de análisis

CargoNúmero de lote
Anti-MyoD, clone 5F11 - 2370185 2370185
Anti-MyoD, clone 5F11 - 2291000 2291000
Anti-MyoD, clone 5F11 - 2291000A 2291000A
Anti-MyoD, clone 5F11 - 3230534 3230534
Anti-MyoD, clone 5F11 - 3506605 3506605
Anti-MyoD, clone 5F11 - 3766698 3766698
Anti-MyoD, clone 5F11 - 3819978 3819978
Anti-MyoD, clone 5F11 - 3928034 3928034
Anti-MyoD, clone 5F11 - 4130129 4130129
Anti-MyoD, clone 5F11 - 4197602 4197602
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