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NE1019 Anti-Myelin Basic Protein Mouse mAb (SMI-99)

This Anti-Myelin Basic Protein Mouse mAb (SMI-99) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Frozen Sections, Paraffin Sections for the detection of Myelin Basic Protein.

Anti-Myelin Basic Protein Mouse mAb (SMI-99): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
NE1019
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
Gp, MaMMonoclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
NE1019-100UL Ampolla de plást. 100 ul
Description
OverviewRecognizes the ~21 kDa myelin basic protein in rat brain tissue. Does not recognize the 14 kDa form. Recognizes most mammalian species.
Catalogue NumberNE1019
Brand Family Calbiochem®
Application Data
Detection of rat myelin basic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019) (1:1000). Detection: fluorescence (green) with Hoechst 33342 counterstain.
References
ReferencesEvers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197.
Shin, R.W., et al. 1991. Lab. Invest. 64, 693.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlRat brain
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (1:1000)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000)
Frozen Sections (1:1000)
Paraffin Sections (1:1000)
Application CommentsRecognizes MBP from most mammalian species. Does not recognize the ~14 kDa form of MBP. Detects developing and adult myelin and developing oligodendrocytes. Also distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue sections. In combination with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenhuman myelin basic protein
ImmunogenHuman
EpitopeWithin amino acids 131-136
CloneSMI-99
HostMouse
IsotypeIgG2b
Species Reactivity
  • Guinea Pig
  • Mammals
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
NE1019-100UL 04055977209884

Documentation

Anti-Myelin Basic Protein Mouse mAb (SMI-99) Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-Myelin Basic Protein Mouse mAb (SMI-99) Certificados de análisis

CargoNúmero de lote
NE1019

Referencias bibliográficas

Visión general referencias
Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197.
Shin, R.W., et al. 1991. Lab. Invest. 64, 693.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-July-2008 JSW
ApplicationELISA (1:1000)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000)
Frozen Sections (1:1000)
Paraffin Sections (1:1000)
Application Data
Detection of rat myelin basic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019) (1:1000). Detection: fluorescence (green) with Hoechst 33342 counterstain.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~21 kDa myelin basic protein (MBP).
BackgroundThe myelin sheath is essential for rapid and effective propagation of action potential within the axon. Mutations identified in several myelin-related genes reportedly lead to severe demyelinating neuropathies. Recent evidence also suggests that the autoimmune disease Multiple Sclerosis (MS) is mediated by T cells with specificity for antigens in the myelin sheath. Although myelin in the central nervous system (CNS) and peripheral nervous system (PNS) is structurally similar, it is chemically distinct since oligodendrocytes form myelin in the CNS whereas Schwann cells generate PNS myelin. Myelin proteolipid protein (PLP) is the predominant integral membrane protein in mammalian CNS myelin. In contrast, myelin basic protein (MBP) is localized to the myelin cytoplasm and is highly conserved among species. Both MBP and PLP mediate myelin compaction, whereas thickness is regulated by MBP. Several other myelin proteins have been identified to play distinct or partially overlapping functions in the formation and maintenance of the myelin sheath, including P0, myelin-associated glycoprotein (MAG), peripheral myelin protein (PMP22), and Connexin 32 (Cx32). The development of the myelin sheath has been a good model for understanding the mechanisms of neuron-glia interaction.
HostMouse
Immunogen speciesHuman
Immunogenhuman myelin basic protein
EpitopeWithin amino acids 131-136
CloneSMI-99
IsotypeIgG2b
Speciesnot chicken, guinea pig (weakly), mammalian, not porcine
Positive controlRat brain
FormLiquid
FormulationIn PBS.
Preservative≤0.1% sodium azide
CommentsRecognizes MBP from most mammalian species. Does not recognize the ~14 kDa form of MBP. Detects developing and adult myelin and developing oligodendrocytes. Also distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue sections. In combination with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesEvers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197.
Shin, R.W., et al. 1991. Lab. Invest. 64, 693.