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ABF1059 Anti-MxB Antibody

This Anti-MxB Antibody is validated for use in Western Blotting for the detection of MxB.

Anti-MxB Antibody : Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: MX2 UniProt Number: P20592
ABF1059
100 µL  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbSerumPolyclonal Antibody
Description
Catalogue NumberABF1059
DescriptionAnti-MxB Antibody
Alternate Names
  • Interferon-induced GTP-binding protein Mx2
  • Interferon-regulated resistance GTP-binding protein MxB
  • Myxovirus resistance protein 2
  • p78-related protein
  • MxB
Background InformationInterferon-induced GTP-binding protein Mx2 (UniProt P20592; also known as Interferon-regulated resistance GTP-binding protein MxB, Myxovirus resistance protein 2, p78-related protein, Second interferon-induced protein p78) is encoded by the MX2 (also known as MXB) gene (Gene ID 4600) in human. The interferon-inducible myxovirus resistance (Mx) proteins belong to the family of large GTPases and are highly homologous with dynamins within their GTP-binding domain. MxA/Mx1 is cytosolic, while two MxB/Mx forms exist, a 78 kDa nuclear form and a 76 kDa cytosolic form lacking the N-terminal nuclear localization signal (NLS). Human MxA protein mediates resistance to influenza and several other viruses, while human MxB is also reported to inhibit HIV-1 infection by reducing the level of integrated viral DNA. In addition, HIV-1 with Ala88 mutation in the capsid protein (CA) is found to escape MxB restriction, as a result of impaired CA interaction with the host peptidylprolyl isomerase cyclophilin A (CypA). Likewise, CypA deletion results in the loss of MxB inhibition of HIV-1.
References
Product Information
FormatSerum
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-MxB Antibody is validated for use in Western Blotting for the detection of MxB.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected IFNalpha-2b-induced MxB/Mx2 expression in U87-MG human glioblastoma cells and SupT1 human T lymphoblast cells (Liu, Z., et al. (2013). Cell Host Microbe. 14(4):398-410).
Western Blotting Analysis: A representative lot detected doxycycline-induced MxB/Mx2 expression using virally transformed human T lymphoblast SupT1 cells that stably expresses the reverse tetracycline-controlled transactivator (rtTA) protein and carries the MxB gene under the tetracycline-responsive promoter element/TRE (Liu, Z., et al. (2013). Cell Host Microbe. 14(4):398-410).
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the Dynamin-type G domain of human MxB.
EpitopeDynamin-type G domain
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityExpected to react with both spliced isoforms.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Primate based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MX2
Purification MethodUnpurified
UniProt Number
Molecular Weight~78 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in IFN alpha 2b treated SupT1 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antiserum detected IFNalpha-2b-induced MxB/Mx2 expression in human T lymphoblast SupT1 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
ABF1059 04055977288001

Documentation

Anti-MxB Antibody Certificados de análisis

CargoNúmero de lote
Anti-MxB - 3683786 3683786
Anti-MxB -Q2607332 Q2607332

Referencias bibliográficas

Visión general referenciasPub Med ID
The interferon-inducible MxB protein inhibits HIV-1 infection.
Liu, Z; Pan, Q; Ding, S; Qian, J; Xu, F; Zhou, J; Cen, S; Guo, F; Liang, C
Cell host & microbe  14  398-410  2013

Mostrar resumen 		24055605 24055605

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