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444905 Anti-Mast Cell Tryptase Mouse mAb (AA1)

This Anti-Mast Cell Tryptase Mouse mAb (AA1) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of Mast Cell Tryptase.

Anti-Mast Cell Tryptase Mouse mAb (AA1): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
444905
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
Ca, Fe, H, MkMMonoclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
444905-100UG Ampolla de plást. 100 μg
Description
OverviewRecognizes the 32-38 kDa α- and β-isoforms of tryptase in mast cells.
Catalogue Number444905
Brand Family Calbiochem®
Application Data
Detection of human mast cell tryptase by immunohistochemistry. Sample: Human tonsil. Priamry antibody: Anti-Mast Cell Tryptase Mouse mAb (AA1) (Cat. No. 444905) (1:2000). Detection: fluorescence.
References
ReferencesSommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75.
Little, S.S., and Johnson, D.A. 1995. Biochem. J. 307, 341.
Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol. 87, 111.
Walls, A.F., et al. 1990. J. Pathol. 162, 119.
Wall, A.F., et al. 1990. Clin. Exp. Allergy 20, 581.
Product Information
FormLiquid
FormulationIn 10 mM PBS, pH 7.4.
Positive controlCat, dog, human, or monkey mast cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesFrozen Sections (0.06-0.12 µg/ml)
Immunoblotting (1 µg/ml)
Paraffin Sections (0.06-0.12 µg/ml, heat pre-treatment required, see comments)
Application CommentsStaining of formalin-fixed paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogennative, human mast cell, tryptase
ImmunogenHuman
CloneAA1
HostMouse
IsotypeIgG₁
Species Reactivity
  • Canine
  • Feline
  • Human
  • Monkey
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
444905-100UG 04055977186208

Documentation

Anti-Mast Cell Tryptase Mouse mAb (AA1) Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-Mast Cell Tryptase Mouse mAb (AA1) Certificados de análisis

CargoNúmero de lote
444905

Referencias bibliográficas

Visión general referencias
Sommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75.
Little, S.S., and Johnson, D.A. 1995. Biochem. J. 307, 341.
Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol. 87, 111.
Walls, A.F., et al. 1990. J. Pathol. 162, 119.
Wall, A.F., et al. 1990. Clin. Exp. Allergy 20, 581.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-October-2008 JSW
ApplicationFrozen Sections (0.06-0.12 µg/ml)
Immunoblotting (1 µg/ml)
Paraffin Sections (0.06-0.12 µg/ml, heat pre-treatment required, see comments)
Application Data
Detection of human mast cell tryptase by immunohistochemistry. Sample: Human tonsil. Priamry antibody: Anti-Mast Cell Tryptase Mouse mAb (AA1) (Cat. No. 444905) (1:2000). Detection: fluorescence.
DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~32-38 kDa α- and β-isoforms of tryptase in mast cells.
BackgroundMast cells are tissue bound cells that normally are distributed throughout connective tissues. They are involved in inflammatory processes as important effector and regulatory cells. Mast cells are known to accumulate at sites of inflammation, such as asthma, allergic rhinitis, and rheumatoid arthritis. Mast cells contain preformed chemical mediators including histamine, chymase, carboxypeptidase and proteolytic tryptase. Mast cells of the skin and lung contain large amounts of tryptase, suggesting this enzyme has a major role in mast cell-mediated events. Human mast cell tryptase has the potential to be used as marker for mast cell activation and as a mediator of inflammation.
HostMouse
Immunogen speciesHuman
Immunogennative, human mast cell, tryptase
CloneAA1
IsotypeIgG₁
Speciescanine, feline, human, monkey
Positive controlCat, dog, human, or monkey mast cells
FormLiquid
FormulationIn 10 mM PBS, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsStaining of formalin-fixed paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Variables associated with assay conditions will dictate the optimal working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesSommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75.
Little, S.S., and Johnson, D.A. 1995. Biochem. J. 307, 341.
Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol. 87, 111.
Walls, A.F., et al. 1990. J. Pathol. 162, 119.
Wall, A.F., et al. 1990. Clin. Exp. Allergy 20, 581.