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NA27 Anti-MSH2 (Ab-2) Mouse mAb (FE11)

NA27
Purchase on Sigma-Aldrich

Descripción

Tabla espec. clave

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
NA27-100UG Ampolla de plást. 100 μg
Description
OverviewRecognizes the ~100 kDa MSH2 protein in HCT116 and SW480 cells and in colon tissue.
Catalogue NumberNA27
Brand Family Calbiochem®
SynonymsAnti-Mismatch Repair Protein 2
References
ReferencesBronner, C.E., et al. 1994. Nature 368, 258.
Papadopoulos, N., et al. 1994. Science 263, 1625.
Peltomäki, P.T. 1994. Annals Med. 26, 215.
Fishel, R., et al. 1993. Cell 75, 1027.
Leach, F.S., et al. 1993. Cell 75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics 5, 279.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Negative controlLoVo cells
Positive controlHCT116 or SW480 cells or colon tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting, Frozen Sections Leach, F.S., et al. 1996. Cancer Res. 56, 235. Paraffin Sections Thibodeau, S.N., et al. 1996. Cancer Res. 56, 4836.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
Application NotesFrozen Sections (2 µg/ml, see application references)
Immunoblotting (0.2 µg/ml, see application references)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (2 µg/ml, heat or proteinase K pre-treatment required, see application references)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena C-terminal fragment of human MSH2
ImmunogenHuman
CloneFE11
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Número de referencia GTIN
NA27-100UG 07790788053857