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MABE1036 Anti-MED15 Antibody, clone 9D3.1

Anti-MED15 Antibody, clone 9D3.1 is an antibody against MED15 for use in Western Blotting.

Anti-MED15 Antibody, clone 9D3.1 : Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: MED15, ARC105, CTG7A, PCQAP, TIG1, TNRC7 UniProt Number: Q96RN5
MABE1036
100 μg  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1036
DescriptionAnti-MED15 Antibody, clone 9D3.1
Alternate Names
  • Mediator of RNA polymerase II transcription subunit 15
  • Activator-recruited cofactor 105 kDa component
  • ARC105
  • CTG repeat protein 7a
  • Mediator complex subunit 15
  • Positive cofactor 2 glutamine/Q-rich-associated protein
  • PC2 glutamine/Q-rich-associated protein
  • TPA-inducible gene 1 protein
  • TIG-1
  • Trinucleotide repeat-containing gene 7 protein
Background InformationMediator of RNA polymerase II transcription subunit 15 (UniProt Q96RN5; also known as Activator-recruited cofactor 105 kDa component, ARC105, CTG repeat protein 7a, Mediator complex subunit 15, PC2 glutamine/Q-rich-associated protein, Positive cofactor 2 glutamine/Q-rich-associated protein, TIG-1, TPA-inducible gene 1 protein, Trinucleotide repeat-containing gene 7 protein) is encoded by the MED15 (also known as ARC105, CTG7A, PCQAP, TIG1, TNRC7) gene (Gene ID 51586) in human. The Mediator complex is an essential transcriptional cofactor of RNA polymerase II (Pol II). The Mediator complex is composed of the CDK/cyclin, the head, the middle, and the tail modules. The tail module consists of eight subunits (MED2/MED29, MED3/MED27, MED5/MED24, MED14, MED15, MED16, MED23, and MED25) and mediates the recruitment of various transcriptional regulators. Human MED15 contains an N-terminal KIX domain (a.a. 9-73) that mediates MED15 binding to the sterol regulatory element binding protein (SREBP) and is important for MED15 function in cholesterol and fatty acid homeostasis. Similar to its yeast counterpart (yeast Gal11), human MED15 is found to colocalize with TFIIE and TFIIF, most likely promoting transcriptional activation in collaboration with these two transcription factors.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MED15 Antibody, clone 9D3.1 is an antibody against MED15 for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant human MED15 internal fragment.
EpitopeInternal (C-terminal half).
Clone9D3.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with all three spliced isoforms reported by UniProt (Q96RN5).
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MED15
  • ARC105
  • CTG7A
  • PCQAP
  • TIG1
  • TNRC7
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~105 kDa observed. Target band size appears larger than the calculated molecular weights of 86.8 kDa (isoform 1), 82.6 kDa (isoform 2), and 75.9 kDa (isoform 3).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected MED15 in 10 µg of K562 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABE1036 04055977314250