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MABS814 Anti-MAP2K4, clone 5H4 Antibody

Anti-MAP2K4, clone 5H4 Antibody is an antibody against MAP2K4 for use in Western Blotting, Immunohistochemistry, Flow Cytometry, ELISA.

Anti-MAP2K4, clone 5H4 Antibody: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: MAP2K4, JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1 UniProt Number: P45985
MABS814
100 µL   
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, FC, ELISAMAscitesMonoclonal Antibody
Description
Catalogue NumberMABS814
Replaces04-382
DescriptionAnti-MAP2K4, clone 5H4 Antibody
Alternate Names
  • Dual specificity mitogen-activated protein kinase kinase 4
  • MAP kinase kinase 4
  • MAPKK 4
  • MAP2K4
Background InformationDual specificity mitogen-activated protein kinase kinase 4 (MAP2K4) is a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
References
Product Information
FormatAscites
PresentationMouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-MAP2K4, clone 5H4 Antibody is an antibody against MAP2K4 for use in Western Blotting, Immunohistochemistry, Flow Cytometry, ELISA.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Flow Cytometry
  • ELISA
Application NotesImmunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected MAP2K4 in muscle tissue.

Flow Cytometry Analysis (FC): A 1:200-400 dilution from a representative lot detected MAP2K4 in K562 cells.

ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Biological Information
ImmunogenPurified recombinant fragment of human MAP2K4 expressed in E. Coli.
Clone5H4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • MAP2K4
  • JNKK1
  • MEK4
  • MKK4
  • PRKMK4
  • SEK1
  • SERK1
  • SKK1
Purification MethodUnpurified
UniProt Number
Molecular Weight~40 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2, K562 and HEK293 cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected MAP2K4 in HepG2, K562 and HEK293 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABS814 04055977172324