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ABE498 Anti-Lysine-specific demethylase 7 Antibody

Detect Lysine-specific demethylase 7 using this rabbit polyclonal antibody, Anti-Lysine-specific demethylase 7 Antibody validated for use in western blotting.

Anti-Lysine-specific demethylase 7 Antibody: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: JHDM1D, KDM7 UniProt Number: Q6ZMT4
ABE498
100 µg  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE498
DescriptionAnti-Lysine-specific demethylase 7 Antibody
Alternate Names
  • Lysine-specific demethylase 7
  • JmjC domain-containing histone demethylation protein 1D
Background InformationLysine-specific demethylase 7, also known as JmjC domain-containing histone demethylation protein 1D, and encoded by the gene JHDM1D/KDM7/KIAA1718, is an important histone demethylase particularly for brain development. JHDM1D specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. JHDM1D also specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. JHDM1D also demethylates H3K9me2 in absence of H3K4me3 and JHDM1D has activity toward H4K20Me1 only when the nucleosome is used as a substrate and when not the histone octamer is used as substrate. JHDM1D is located in the nucleus and widely expressed but lowered expression is observed in glial cells and lymphoid cells. JHDM1D demonstrates tumor suppression by regulating angiogenesis factors when overexpression under starvation conditions in certain cancers, thus JHDM1D is being examined as a potential anti-cancer target.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Lysine-specific demethylase 7 using this rabbit polyclonal antibody, Anti-Lysine-specific demethylase 7 Antibody validated for use in western blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Lysine-specific demethylase 7.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody is expected to cross react with two known isoforms of Lysine-specific demethylase 7 based upon immunogen design.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteDemonstrated to react with Human and Mouse. Predicted to react with Canine, Mouse, Rhesus Macaque, Chimpanzee Giant Panda, and Marmoset based upon sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • JHDM1D
  • KDM7
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~110 kDa observed. Uniprot describes two isoforms at 107 kDa (isoform 1) and 92 kDa (isoform 2).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Lysine-specific demethylase 7 in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
ABE498 04053252930867