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MABC1193 Anti-LC3A/B Antibody, clone 4F11.1

Anti-LC3A/B, clone 4F11.1, Cat. No. MABC1193, is a mouse monoclonal antibody that detects LC3A/B and has been tested for use in Western Blotting.

Anti-LC3A/B Antibody, clone 4F11.1: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Apoptosis & Cancer Gene Symbol: MAP1LC3B, MAP1ALC3 UniProt Number: Q9GZQ8
MABC1193
100 μg  
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1193
DescriptionAnti-LC3A/B Antibody, clone 4F11.1
Alternate Names
  • Microtubule-associated proteins 1A/1B light chain 3B
  • Autophagy-related protein LC3 B
  • Autophagy-related ubiquitin-like modifier LC3 B
  • MAP1 light chain 3-like protein 2
  • MAP1A/MAP1B light chain 3 B
  • MAP1A/MAP1B LC3 B
  • Microtubule-associated protein 1 light chain 3 beta
Background InformationMicrotubule-associated proteins 1A/1B light chain 3B (UniProt: Q9GZQ8; also known as Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta) is encoded by the MAP1LC3B (also known as MAP1ALC3) gene (Gene ID: 81631) in human. Autophagy-related protein LC3 A/B is an ubiquitin-like modifier that is involved in formation of autophagosomal vacuoles (autophagosomes). It plays a role in mitophagy that contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess reactive oxygen species production. It contains a microtubule-associated proteins 1A/1B light chain 3B (aa 2-120) and a propeptide region (aa 121-125) that is cleaved off in mature form. The precursor molecule is cleaved by ATG4B (between aa 120-121) to form the cytosolic form LC3-I, which is activated by ATG7 and then transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) to generate the membrane-bound LC3-II. Phosphorylation at threonine12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Autophagy-related protein LC3 B is found to be most abundant in heart, brain, skeletal muscle and testis.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-LC3A/B, clone 4F11.1, Cat. No. MABC1193, is a mouse monoclonal antibody that detects LC3A/B and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to amino acids 2-74 of human Autophagy-related protein LC3 B.
Clone4F11.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 4F11.1 detects Autophagy-related protein LC3 B in human cells.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MAP1LC3B
  • MAP1ALC3
Purification MethodProtein G purified
UniProt Number
Molecular Weight~15 kDa observed; 14.69 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cells treated with 50 µM chloroquine.

Western Blotting Analysis: 1 µg/mL of this antibody detected LC3A/B in 10 µg of lysate from HeLa cells treated with 50 µM chloroquine.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABC1193 04054839339639