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MABT1536-100UG Anti-KIF2 Antibody, clone 2B12.C1

Anti-KIF2, clone 2B12.C1, Cat. No. MABT1536, is a mouse monoclonal antibody that detects Kinesin-like protein KIF2A and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

MABT1536-100UG
100 μg  
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue NumberMABT1536-100UG
DescriptionAnti-KIF2 Antibody, clone 2B12.C1
Alternate Names
  • Kinesin-2
  • Kinesin-like protein KIF2A
Background InformationKinesin-like protein KIF2A (UniProt: P28740; also known as Kinesin-2, KIF2A) is encoded by the Kif2a (also known as Kif2, Kns2) gene (Gene ID: 16563) in murine species. KIF2A is a member of the Kinesin-13 family that acts as a central regulator of the microtubule cytoskeleton in neurons. Proteins in this family include KIF2A, KIF2B, KIF2C/ MCAK, and KIF24. KIF2A acts as an ATP-dependent microtubule destabilizer and regulates cell migration, axon elongation, and pruning in the developing nervous system. KIF2A is highly phosphorylated in the brain and has many phosphorylation sites (Thr186/Thr187; Thr439/Ser440/Ser441, Ser470 and Ser488) that are shown to be specific targets of p21-activated kinase 1 (PAK1). Phosphorylation is reported to transform normal KIF2A into accelerating KIF2A (A-KIF2A) or braking KIF2A (B-KIF2A) that regulate microtubule dynamics and neuron morphology in response to extracellular signals. It has been shown that brain-derived neurotrophic factor (BDNF) stimulates PAK1 and CDK5 kinases, which can reduce microtubule polymerization activity of KIF2A via B-type phosphorylation. This results in enhanced outgrowth of neural processes. On the other hand, lysophosphatidic acid (LPA) can induce ROCK2 kinase activity that suppresses neurite outgrowth from round cells via A-type phosphorylation. (Ref.: Ogawa, T., and Hirokawa, N. (2015). Cell Reports 12(11); 1774-1788; Homma, N., et al. (2018). eLife.7:e30935).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-KIF2, clone 2B12.C1, Cat. No. MABT1536, is a mouse monoclonal antibody that detects Kinesin-like protein KIF2A and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected KIF2 in mouse testis tissue sections.

Western Blotting Analysis: 1 µg/mL from a representative lot detected KIF2 in mouse brain tissue lysate.
Biological Information
ImmunogenT7-tagged full-length mouse recombinant Kinesin-like protein KIF2A.
Clone2B12.C1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 2B12.C1 is a mouse monoclonal antibody that detects Kinesin-like protein KIF2A in murine species.
IsotypeIgGκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Kif2a
  • Kif2
  • Kns2
Purification MethodProtein G purified
UniProt Number
Molecular Weight79.75 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse brain tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected KIF2 in mouse brain tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABT1536-100UG 04054839007187