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05-535 Anti-IKKβ Antibody, clone 10AG2

05-535
100 µg  
Purchase on Sigma-Aldrich

Ofertas especiales

Descripción

Replacement Information

Ofertas especiales

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-535
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-IKKβ Antibody, clone 10AG2
Alternate Names
  • I-kappa-B kinase 2
  • I-kappa-B-kinase beta
  • Nuclear factor NF-kappa-B inhibitor kinase beta
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
  • inhibitor of nuclear factor kappa B kinase beta subunit
Background InformationIKK beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK beta has been shown to activate NF-kB and phosphorylate IKB alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK-1.
References
Product Information
FormatPurified
Control
  • Jurkat cell lysate and human cervical carcinoma.
    Included Positive Antigen Control: Catalog # 12-303, Jurkat cell extract. Use 20 µg per lane for minigels.
PresentationPurified mouse monoclonal IgG1 in buffer containing PBS and 0.05% sodium azide. Frozen solution.
Quality LevelMQ100
Applications
ApplicationAnti-IKKβ Antibody, clone 10AG2 detects level of IKKβ & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenHis-tagged, full length human IKKβ/IKK2. Clone: 10AG2.
Clone10AG2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes IKKβ/IKK2, Mr 87 kDa.
IsotypeIgG1
Species Reactivity
  • Human
  • Rat
  • Mouse
Species Reactivity NoteProven to react with human, mouse and rat. Other species not tested.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryNFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
Gene Symbol
  • IKBKB
  • IKK-beta
  • NFKBIKB
  • IKK2
  • FLJ40509
  • IKKB
  • MGC131801
  • IKK-B
  • IkBKB
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O14920 # Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3 (By similarity).
SIZE: 756 amino acids; 86564 Da
SUBUNIT: Preferentially found as a heterodimer with IKK-alpha but also as a homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. The tripartite complex can also bind to MEKK1, MAP3K14/NIK, IKAP and IKB-alpha-p65-p50 complex. Phosphorylated IKB-alpha is further released from the complex. Found in a complex composed of NCOA2, NCOA3, IKKA, IKBKG and CREBBP. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6. May interact with MAVS/IPS1. Interacts with NALP2. Interacts with Yersinia yopJ.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
PTM: Upon cytokine stimulation, phosphorylated on Ser-177 and Ser- 181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response. & Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, which blocks the I-kappa-B signaling pathway.
SIMILARITY: SwissProt: O14920 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. IkappaB kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight87 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by western blot on human Jurkat RIPA cell lysate, human A431, HeLa nuclear extract, mouse 3T3/A31 or rat PC-12 RIPA cell lysates.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected IKK/IKK2 in human HeLa cell lysate. A previous lot was tested in human Jurkat RIPA cell lysate, A431, extract, mouse 3T3/A31 and rat PC-12 RIPA cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
05-535 08436037125331

Documentation

Anti-IKKβ Antibody, clone 10AG2 Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-IKKβ Antibody, clone 10AG2 Certificados de análisis

CargoNúmero de lote
Anti-IKK#946;, clone 10AG2 - DAM1749919 DAM1749919
Anti-IKK#946;, clone 10AG2 - 2137999 2137999
Anti-IKK, clone 10AG2 - 2064509 2064509
Anti-IKK, clone 10AG2 - 2203116 2203116
Anti-IKK, clone 10AG2 - 2290926 2290926
Anti-IKK, clone 10AG2 - DAM1442517 DAM1442517
Anti-IKK, clone 10AG2 - DAM1734752 DAM1734752
Anti-IKK, clone 10AG2 - DAM1774677 DAM1774677
Anti-IKK, clone 10AG2 - JBC1872234 JBC1872234
Anti-IKKBeta, clone 10AG2 - DAM1543628 DAM1543628

Referencias bibliográficas

Visión general referenciasAplicación EspeciePub Med ID
IKK is a therapeutic target in KRAS-Induced lung cancer with disrupted p53 activity.
Bassères, DS; Ebbs, A; Cogswell, PC; Baldwin, AS
Genes & cancer  5  41-55  2014

Mostrar resumen
Western Blotting24955217 24955217
Intestinal tumorigenesis initiated by dedifferentiation and acquisition of stem-cell-like properties.
Schwitalla, S; Fingerle, AA; Cammareri, P; Nebelsiek, T; Göktuna, SI; Ziegler, PK; Canli, O; Heijmans, J; Huels, DJ; Moreaux, G; Rupec, RA; Gerhard, M; Schmid, R; Barker, N; Clevers, H; Lang, R; Neumann, J; Kirchner, T; Taketo, MM; van den Brink, GR; Sansom, OJ; Arkan, MC; Greten, FR
Cell  152  25-38  2013

Mostrar resumen
23273993 23273993
Transcription of Tnfaip3 is regulated by NF-κB and p38 via C/EBPβ in activated macrophages.
Lai, TY; Wu, SD; Tsai, MH; Chuang, EY; Chuang, LL; Hsu, LC; Lai, LC
PloS one  8  e73153  2013

Mostrar resumen
Western BlottingMouse24023826 24023826
IκB kinase β is required for activation of NF-κB and AP-1 in CD3/CD28-stimulated primary CD4(+) T cells.
Lupino, E; Ramondetti, C; Piccinini, M
Journal of immunology (Baltimore, Md. : 1950)  188  2545-55  2011

Mostrar resumen
22331067 22331067
zVAD-induced necroptosis in L929 cells depends on autocrine production of TNFα mediated by the PKC-MAPKs-AP-1 pathway.
Wu, YT; Tan, HL; Huang, Q; Sun, XJ; Zhu, X; Shen, HM
Cell death and differentiation  18  26-37  2010

Mostrar resumen
20539307 20539307
Crystal structure of inhibitor of κB kinase β.
Xu, G; Lo, YC; Li, Q; Napolitano, G; Wu, X; Jiang, X; Dreano, M; Karin, M; Wu, H
Nature  472  325-30  2010

Mostrar resumen
Western Blotting21423167 21423167
NF-κB essential modulator (NEMO) interaction with linear and lys-63 ubiquitin chains contributes to NF-κB activation.
Hadian, K; Griesbach, RA; Dornauer, S; Wanger, TM; Nagel, D; Metlitzky, M; Beisker, W; Schmidt-Supprian, M; Krappmann, D
The Journal of biological chemistry  286  26107-17  2010

Mostrar resumen
21622571 21622571
Requirement of the NF-kappaB subunit p65/RelA for K-Ras-induced lung tumorigenesis.
Bassères, DS; Ebbs, A; Levantini, E; Baldwin, AS
Cancer research  70  3537-46  2009

Mostrar resumen
20406971 20406971
High-fat feeding increases insulin receptor and IRS-1 coimmunoprecipitation with SOCS-3, IKKalpha/beta phosphorylation and decreases PI-3 kinase activity in muscle.
Yaspelkis, BB; Kvasha, IA; Figueroa, TY
American journal of physiology. Regulatory, integrative and comparative physiology  296  R1709-15  2009

Mostrar resumen
Western Blotting19386987 19386987
LPS induces KH-type splicing regulatory protein-dependent processing of microRNA-155 precursors in macrophages.
Tina Ruggiero, Michele Trabucchi, Francesca De Santa, Simona Zupo, Brian D Harfe, Michael T McManus, M Geoff Rosenfeld, Paola Briata, Roberto Gherzi
The FASEB journal : official publication of the Federation of American Societies for Experimental Biology  23  2898-908  2009

Mostrar resumen
Immunoblotting (Western)Mouse19423639 19423639

Folleto

Cargo
Pathways and Biomarkers of Toll-like Receptor (TLR) Signaling