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AB10212 Anti-IHH Antibody

Detect IHH using this Anti-IHH Antibody validated for use in WB.

Anti-IHH Antibody: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Stem Cell Research Research Sub-Category: Hematopoietic Stem Cells Gene Symbol: BDA1, HHG-2, HHG2 UniProt Number: P97812
AB10212
100 µg  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, R, Ca, Eq, BWBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberAB10212
DescriptionAnti-IHH Antibody
Alternate Names
  • Indian hedgehog homolog
Background InformationIndian hedgehog (IHH) is a member of the hedgehog family of signaling molecules that play diverse roles in patterning early embryonic events. The hedgehog family of morphogens are regulators of cell proliferation, differentiation, and cell-cell communication, and have been shown to have important roles in organogenesis, spermatogenesis, stem cell maintenance, and oncogenesis. The Indian hedgehog (IHH) signal has an essential role in inducing hematopoietic tissue during embryogenesis, and is synthesized by chondrocytes and thymic epithelial cells. In addition to the development of hematopoietic cells, IHH is also responsible for regulating the rate of bone growth and cartilage differentiation during skeletal morphogenesis and intestinal epithelium.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal in buffer containing PBS with 0.09% sodium azide.
Applications
ApplicationDetect IHH using this Anti-IHH Antibody validated for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern Blotting: Recommended working dilutions are 1:250 – 1:400.
Biological Information
ImmunogenSynthetic mouse IHH peptide, a.a. 146-162.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody is generated from rabbits immunized with a KLH conjugated synthetic peptided selected from mouse IHH.
Species Reactivity
  • Mouse
  • Human
  • Rat
  • Canine
  • Horse
  • Bovine
Species Reactivity NoteReacts with mouse. Comparison of sequence homology suggests that this antibody may also react with human, rat, canine, bovine, and equine; however, reactivity with other species has not been tested.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • BDA1
  • HHG-2
  • HHG2
UniProt Number
UniProt SummaryFUNCTION: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).
SUBCELLULAR LOCATION: Indian hedgehog protein N-product: Cell membrane; Lipid-anchor; Extracellular side (By similarity). Note=The N-terminal peptide remains associated with the cell surface (By similarity).
SUBCELLULAR LOCATION: Indian hedgehog protein C-product: Secreted, extracellular space (By similarity). Note=The C-terminal peptide diffuses from the cell (By similarity).
TISSUE SPECIFICITY: In the embryo, detected in the developing gut, the growth zone of cartilage of developing long bones, epithelium and urogenital sinus. In the adult kidney, found in proximal convoluted and proximal straight tubule.
DEVELOPMENTAL STAGE: Detected at 10 dpc in developing gut, at 14.5 days dpc in the cartilage primordium and in the developing urogenital sinus. Expression increases with gestional age in kidney and duodenum, becoming maximal in adulthood.
PTM: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity).
PTM: Cholesterylation is required for N-product targeting to lipid rafts and multimerization (By similarity).
PTM: N-palmitoylation is required for N-product multimerization and full activity (By similarity).
SIMILARITY: Belongs to the hedgehog family.
Stem Cell Type
  • Hematopoietic Stem Cells
Molecular Weight~45 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western blot on mouse heart cytosol lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
AB10212 04053252319747

Documentation

Anti-IHH Antibody Certificados de análisis

CargoNúmero de lote
Anti-IHH - NG1551923 NG1551923