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05-858 Anti-Histone H4 Antibody, pan, clone 62-141-13, rabbit monoclonal

05-858
100 µL  
Purchase on Sigma-Aldrich

Ofertas especiales

Descripción

Ofertas especiales

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Ch, Vrt, Yeast (S. cerevisiae)WB, Mplex, ChIP-seq, ChIPRbPurifiedMonoclonal Antibody
Description
Catalogue Number05-858
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Histone H4 Antibody, pan, clone 62-141-13, rabbit monoclonal
OverviewThis antibody recognizes histone H4 independant of any post-translational modifications.
Alternate Names
  • H4
  • Histone H4
  • H4 histone family, member A
  • histone 1
  • H4a
  • histone cluster 1
  • H4a
Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
Product Information
FormatPurified
Control
  • Acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated).
PresentationPurified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Histone H4 Antibody, pan, clone 62-141-13 is a Rabbit Monoclonal Antibody for detection of Histone H4 also known as Histone H4 & has been validated in ChIP, WB, Mplex, ChIP-seq.
Key Applications
  • Western Blotting
  • Multiplexing
  • ChIP-seq
  • Chromatin Immunoprecipitation (ChIP)
Application NotesEpitope Mapping (using Luminex®):
1:250-1:4,000 serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the amino-terminus of Histone H4 conjugated to Luminex® microspheres. The antibody recognized both modified and unmodified peptides (See Figure B Page 2).

Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).
Biological Information
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28.
Epitopea.a. 25-28
Cloneclone 62-141-13
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes Histone H4, Mr 10 kDa.
IsotypeIgG
Species Reactivity
  • Human
  • Chicken
  • Vertebrates
  • Yeast (S. cerevisiae)
Species Reactivity NoteHuman, chicken and yeast. Broad species cross-reactivity expected based on sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Gene Symbol
  • HIST1H4A H4/J HIST1H4F HIST1H4L H4FN H4FA H4FH
  • HIST1H4H
  • H4F2
  • H4FM
  • H4/A
  • H4FK
  • H4FG
  • HIST2H4
  • H4FB
  • H4/K
  • HIST1H4I
  • H4/N
  • HIST1H4B
  • H4FE
  • H4/M
  • H4/a
  • HIST1H4E
  • HIST4H4
  • HIST2H4A
  • H4FC
  • H4FI
  • H4/H
  • HIST1H4C
  • H4/G
  • HIST1H4K
  • H4FJ
  • H4FD
  • H4/I
  • H4/B
  • H4/D
  • H4/C
  • HIST1H4J
  • HIST1H4D
  • H4/E
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 103 amino acids; 11367 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
Molecular Weight10 kDa
Product Usage Statements
Quality AssuranceRoutinely evaluated by western blot.

Western Blot Analysis:
A 1:30,000-1:100,000 dilution of this lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Número de referencia GTIN
05-858 04053252404337