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05-100-I Anti-HDAC1 Antibody, clone 2E10

05-100-I
100 µL  
Purchase on Sigma-Aldrich

Ofertas especiales

Descripción

Replacement Information

Ofertas especiales

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IPMPurifiedMonoclonal Antibody
Description
Catalogue Number05-100-I
Replaces05-100
Brand Family Upstate
DescriptionAnti-HDAC1 Antibody, clone 2E10
Alternate Names
  • HDAC1
  • Histone deacetylase 1
  • HD1
Background InformationHistone deacetylase 1 is a nuclear enzyme encoded by the Hdac1 gene (also known as HD1, RPD3, MommeD5, Hdac1-ps) in mouse and HDAC1 (also known as GoON-10, RPD3L1) gene in human. Hdac1 plays an important role in epigenetic regulation of gene expression by catalyzing the hydrolysis of N(6)-acetylated histone lysine residues. Hdac1 is also known to deacetylate p53 and thereby downregulate p53 activity.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-HDAC1 Antibody, clone 2E10 is an antibody against HDAC1 for use in Western Blotting, Immunoprecipitation.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis A representative lot was used to immunoprecipitate HDAC1 from HeLa cell lysate, followed by immune complex enzyme activity assay using HDAC Assay Kit (Fluorometric Detection), Cat. No. 17-356.
Biological Information
ImmunogenKLH-conjugated, synthetic peptide (C-EEKPEAKGVKEEVKLA) corresponding to amino acids 467-482 of mouse HDAC1. The immunizing sequence is identical in human.
Clone2E10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Hdac1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~55 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected HDAC1 in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
05-100-I 04055977158915

Documentation

Anti-HDAC1 Antibody, clone 2E10 Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-HDAC1 Antibody, clone 2E10 Certificados de análisis

CargoNúmero de lote
Anti-HDAC1, clone 2E10 - 3215508 3215508
Anti-HDAC1, clone 2E10 - 3471510 3471510
Anti-HDAC1, clone 2E10 - 3777265 3777265
Anti-HDAC1, clone 2E10 -Q2544798 Q2544798
Anti-HDAC1, clone 2E10 -Q2550848 Q2550848
Anti-HDAC1, clone 2E10 -Q2557014 Q2557014

Licencias necesarias e Información técnica

Cargo
White Paper: Further considerations of antibody validation and usage.