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MABS87 Anti-GPR177 Antibody, clone YJ5

MABS87
100 µL  
Purchase on Sigma-Aldrich

Ofertas especiales

Descripción

Ofertas especiales

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS87
DescriptionAnti-GPR177 Antibody, clone YJ5
Alternate Names
  • Protein wntless homolog
  • Integral membrane protein GPR177
  • Protein evenness interrupted homolog
  • Putative NF-kappa-B-activating protein 373
  • WLS
  • EVI
Background InformationGpr177 (WLS, EVI), the mouse ortholog of Drosophila WIs, is expressed during mouse embryogenesis. It is essential for the patterning of the anterior-posterior axis during mammalian development and is activated by â-catenin and LEF/TCF-dependent transcription. Upon GPR177 activation, Wnt binds with and modifies the cellular distribution of GPR177, leading to a feedback regulatory mechanism involving Wnt expression and signaling.
Product Information
FormatPurified
Control
  • HeLa cell lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-GPR177 Antibody, clone YJ5 is an antibody against GPR177 for use in WB & IC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GPR177 in HeLa cells (Prof. D.M. Virshup, Program in Cancer and Stem Cell Biology, Duke-NUS Graduate Medical School.) (Also reference Coombs, G.S., et al. (2010). J Cell Sci. 123(19):3357-3367).
Biological Information
ImmunogenHistidine-tagged recombinant protein corresponding to human GPR177.
CloneYJ5
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • WLS
  • C1orf139
  • GPR177
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development.

SUBUNIT STRUCTURE: Interacts with WNT3A. Interacts with WNT1, WNT3 and WNT5A.

SUBCELLULAR LOCATION: Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein.
Molecular Weight~62 kDa observed
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:5,000 dilution of this antibody detected GPR177 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Número de referencia GTIN
MABS87 04053252686450