Millipore Sigma Vibrant Logo
Atención: Nos hemos mudado. Los productos Merck Millipore ya no pueden adquirirse en MerckMillipore.comMás información

MABS508 Anti-GOT1 Antibody, clone 1F5.2

Detect GOT1 using this mouse monoclonal Anti-GOT1 Antibody, clone 1F5.2, Cat. No. MABS508, validated for use in Western Blotting, Immunohistochemistry (Paraffin).

Anti-GOT1 Antibody, clone 1F5.2 : Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: GOT1 UniProt Number: P17174
MABS508
100 μg  
Purchase on Sigma-Aldrich

Ofertas especiales

Descripción

Replacement Information

Ofertas especiales

100% Performance Guaranteed

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS508
DescriptionAnti-GOT1 Antibody, clone 1F5.2
Alternate Names
  • Cysteine aminotransferase, cytoplasmic
  • Cysteine transaminase, cytoplasmic
  • cCAT
  • Glutamate oxaloacetate transaminase 1
  • Transaminase A
  • Aspartate aminotransferase, cytoplasmic
  • cAspAT
  • GOT1
Background InformationAspartate aminotransferase, cytoplasmic (EC 2.6.2.1.; EC 2.6.2.3; UniProt P17174; also known as Aspartate aminotransferase 1, Aspartate transaminase 1, cAspAT, cCAT, Cysteine aminotransferase cytoplasmic, Cysteine transaminase cytoplasmic, Glutamate oxaloacetate transaminase 1, Growth-inhibiting protein 18, Transaminase A) is encoded by the GOT1 (also known as GIG18, ASTQTL1) gene (Gene ID 2805) in human. Aspartate aminotransferase is expressed in many types of cells and tissues and is conserved among prokaryotic and eukaryotic organisms. There exist two highly similar AST forms in mammals, the cytosolic cAST (encoded by the GOT1 gene) and the mitochondrial mAST (encoded by the GOT2 gene). Serum aspartate aminotransferase activity is largely that of cAST/GOT1, and elevated serum AST activity is associated with liver, heart, and muscle injury. AST catalyzes the reversible conversion between aspartate/alpha-ketoglutarate and oxaloacetate/glutamate, increasing oxaloacetate concentration therefore shifts the equilibrium of AST-catalzed reaction toward aspartate/alpha-ketoglutarate and away from glutatmate. Lowering blood glutamate concentration via oxaloacetate i.v. injection prompts an efflux of excess extracellular brain glutamate into the blood, which is reported to allevate the infarct size and edema in a rat model of ischemia induced by transient occlusion of the middle cerebral artery occlusion (MCAO).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect GOT1 using this mouse monoclonal Anti-GOT1 Antibody, clone 1F5.2, Cat. No. MABS508, validated for use in Western Blotting, Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected GOT1 in human liver hepatocytes and in human cerebellum.
Biological Information
ImmunogenGST-tagged Recombinant protein corresponding to the N-terminal region of human GOT1.
EpitopeN-terminal half
Clone1F5.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GOT1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~43 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected GOT1 in 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABS508 04055977181722

Documentation

Anti-GOT1 Antibody, clone 1F5.2 Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-GOT1 Antibody, clone 1F5.2 Certificados de análisis

CargoNúmero de lote
Anti-GOT1, clone 1F5.2 - 3291397 3291397
Anti-GOT1, clone 1F5.2 -Q2583319 Q2583319

Licencias necesarias e Información técnica

Cargo
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.