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ST1543 Anti-GHR Mouse mAb (3A12)

Anti-GHR, mouse monoclonal, clone 3A12, recognizes the GHR protein in HeLa cells. It is validated for use in ELISA, Western blotting, and immunocytochemistry.

Anti-GHR Mouse mAb (3A12): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: Anti-Growth Hormone Receptor

ST1543
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
ST1543-100UG 100 μg
Description
OverviewRecognizes the GHR protein in HeLa cells.
Catalogue NumberST1543
Brand Family Calbiochem®
SynonymsAnti-Growth Hormone Receptor
Application Data
Detection of human GHR by immunoblotting. Sample: Partial recombinant human GHR (immunogen). Primary antibody: Anti-GHR Mouse mAb (3A12) (Cat. No. ST1543) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human GHR by Proximity Ligation Analysis of protein-protein interaction using immunocytochemistry. Sample: HeLa cells. Primary antibodies: Anti-STAT5 (1:200) and Anti-GHR Mouse mAb (3A12) (1:50). Detection: fluorescence with DAPI counterstain.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (1:50)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 19-119 of human GHR, expressed as a GST fusion protein
Clone3A12
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
ST1543-100UG 04055977224245

Documentation

Anti-GHR Mouse mAb (3A12) Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-November-2010 RFH
SynonymsAnti-Growth Hormone Receptor
ApplicationELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (1:50)
Application Data
Detection of human GHR by immunoblotting. Sample: Partial recombinant human GHR (immunogen). Primary antibody: Anti-GHR Mouse mAb (3A12) (Cat. No. ST1543) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human GHR by Proximity Ligation Analysis of protein-protein interaction using immunocytochemistry. Sample: HeLa cells. Primary antibodies: Anti-STAT5 (1:200) and Anti-GHR Mouse mAb (3A12) (1:50). Detection: fluorescence with DAPI counterstain.
DescriptionPurified mouse monoclonal antibody. Recognizes the GHR protein.
BackgroundGHR is a protein that is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. A common alternate allele of this gene, called GHRd3, lacks exon three and has been well-characterized. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 19-119 of human GHR, expressed as a GST fusion protein
Clone3A12
IsotypeIgG2a
Specieshuman
Positive controlHeLa cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review