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ABE281 Anti-EZH1 Antibody

Detect the EZH1 protein using this Anti-EZH1 validated for use in IHC & WB.

Anti-EZH1 Antibody: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins Gene Symbol: EZH1, KIAA0388 UniProt Number: Q92800
ABE281
100 µg  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MIHC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE281
Replaces04-1047
DescriptionAnti-EZH1 Antibody
Alternate Names
  • Histone-lysine N-methyltransferase EZH1
  • ENX-2
  • Enhancer of zeste homolog 1
Background InformationPolycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH1 is the catalytic subunit of the PRC2/EED-EZH1 complex. This complex methylates Lys-27 of histone H3, which leads to transcriptional repression of the affected target gene. EZH1 is required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in maintaining the identity of embryonic stem cells.
References
Product Information
FormatAffinity Purified
Control
  • Mouse embryonic stem cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect the EZH1 protein using this Anti-EZH1 validated for use in IHC & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected EZH1 in human lung and kidney tissues.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human EZH1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody potentially recognizes all isoforms of EZH1.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteDemonstrated to react with Human, and Mouse. Predicted to react with Rat, Rhesus Macaque, and Bovine based on 100% sequence homology. Other homologies: Porcine (94% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • EZH1
  • KIAA0388
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~85 kDa observed. Uniprot states 5 isoforms for EZH1. This antibody potentially recognizes all isoforms. Isoform A is 85,271 Daltons. Isoform B is 86,025 Daltons. Isoform C is 80,699 Daltons. Isoform D is 76,952 Daltons. Isoform E is 68,961 Daltons. (Margueron, 2008).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in mouse embryonic stem cell lysate.

Western Blot Analysis: 0.2 µg/mL of this antibody detected EZH1 in 10 µg of mouse embryonic stem cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
ABE281 04053252819995