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MABE633 Anti-ELK1, clone 3H6D12 Antibody

Anti-ELK1, clone 3H6D12 Antibody is an antibody against ELK1 for use in Western Blotting, Immunohistochemistry.

Anti-ELK1, clone 3H6D12 Antibody: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: ELK1 UniProt Number: P19419
MABE633
100 µL  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABE633
Replaces04-226
DescriptionAnti-ELK1, clone 3H6D12 Antibody
Alternate Names
  • ETS domain-containing protein Elk-1
  • ELK1
Background InformationETS domain-containing protein Elk-1 (ELK1) is a family of member of ETS oncogene family and of the ternary complex factor (TCF) subfamily,which is located on chromosome Xp11.2 and stimulates transcription. binds to purine-rich DNA sequences. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus,It appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.
References
Product Information
FormatAscites
PresentationMouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-ELK1, clone 3H6D12 Antibody is an antibody against ELK1 for use in Western Blotting, Immunohistochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected ELK1 in brain tumor tissue.
Biological Information
ImmunogenPurified recombinant fragment of ELK1 expressed in E. Coli.
Clone3H6D12
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • ELK1
Purification MethodUnpurified
UniProt Number
Molecular Weight~38 and ~75 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in truncated ELK1 recombinant protein and K562 celll lysate.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected ELK1 in truncated ELK1 recombinant protein and K562 celll lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABE633 04055977171815