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ST1513 Anti-EDN3 Mouse mAb (2A6-2A4)

Anti-EDN3, mouse monoclonal, clone 2A6-2A4, recognizes the ~26 kDa EDN3 protein in LNCaP cells. it is validated for use in ELISA and Western blotting.

Anti-EDN3 Mouse mAb (2A6-2A4): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: Anti-Endothelin 3

ST1513
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
ST1513-100UG 100 μg
Description
OverviewRecognizes the ~26 kDa EDN3 protein in LNCaP cells.
Catalogue NumberST1513
Brand Family Calbiochem®
SynonymsAnti-Endothelin 3
Application Data
Detection of human EDN3 by immunoblotting. Sample: Whole cell lysate from LNCaP cells. Primary antibody: Anti-EDN3 Mouse mAb (2A6-2A4) (Cat. No. ST1513) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlLNCaP cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant human EDN3 (aa 1-239), expressed as a GST fusion protein
Clone2A6-2A4
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
ST1513-100UG 04055977224177

Documentation

Anti-EDN3 Mouse mAb (2A6-2A4) Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-November-2010 RFH
SynonymsAnti-Endothelin 3
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human EDN3 by immunoblotting. Sample: Whole cell lysate from LNCaP cells. Primary antibody: Anti-EDN3 Mouse mAb (2A6-2A4) (Cat. No. ST1513) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~26 kDa EDN3 protein.
BackgroundThe protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons.
HostMouse
ImmunogenFull-length, recombinant human EDN3 (aa 1-239), expressed as a GST fusion protein
Clone2A6-2A4
IsotypeIgG₁
Specieshuman
Positive controlLNCaP cells
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review