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ABE551 Anti-E3 ubiquitin-protein ligase UHRF1 Antibody

Detect UHRF1 using this rabbit polyclonal antibody, Anti-E3 ubiquitin-protein ligase UHRF1 Antibody validated for use in western blotting & IHC.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: UHRF1, NP95, RNF106 UniProt Number: Q96T88
ABE551
100 µg  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE551
DescriptionAnti-E3 ubiquitin-protein ligase UHRF1 Antibody
Alternate Names
  • E3 ubiquitin-protein ligase UHRF1
  • Inverted CCAAT box-binding protein of 90 kDa
  • Nuclear protein 95
  • Nuclear zinc finger protein Np95
  • HuNp95
  • hNp95
  • RING finger protein 106
  • Transcription factor ICBP90
  • Ubiquitin-like PHD and RING finger domain-containing protein 1
  • hUHRF1
  • Ubiquitin-like-containing PHD and RING finger domains protein 1
Background InformationUbiquitin-like with PHD and ring finger domains 1 (UHRF1), also known as ICBP90 or Np95, is a multifunctional nuclear protein that may be an important mediator of heterochromatin formation which is indicative of gene repression. UHRF1 contains a set and RING-finger-associated domain that detects hemi-methylated cytosine residues leading to the recruitment of DNMT1 to these residues during the S phase. UHRF1 thereby facilitates the transfer of methylation status to newly synthesized DNA molecules. UHRF1 may also interact with histones and HDAC1 via its PHD domain to promote modifications of histone residues which contribute to heterochromatin formation. UHRF1 may also directly modify histone H3 by ubiquitination. In addition, UHRF1 may contribute to the DNA-repair process though associations with PCNA. Several studies have suggested that overexpression of UHRF1 may contribute to the development of many cancers.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect UHRF1 using this rabbit polyclonal antibody, Anti-E3 ubiquitin-protein ligase UHRF1 Antibody validated for use in western blotting & IHC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected E3 ubiquitin-protein ligase UHRF1 in human breast cancer tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a region near the C-terminus of human E3 ubiquitin-protein ligase UHRF1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Primate, Feline, Bovine, Porcine, Horse, and Canine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • UHRF1
  • NP95
  • RNF106
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~97 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected E3 ubiquitin-protein ligase UHRF1 in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
ABE551 04053252932748