Millipore Sigma Vibrant Logo
Atención: Nos hemos mudado. Los productos Merck Millipore ya no pueden adquirirse en MerckMillipore.comMás información

324393 Anti-Dopamine D₂ Receptor Rabbit pAb

324393
Purchase on Sigma-Aldrich

Descripción

Tabla espec. clave

Species ReactivityHostAntibody Type
H, RRbPolyclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
324393-100UL Ampolla de plást. 100 ul
Description
OverviewRecognizes the dopamine D2 receptor in rat brain. Does not cross-react with other dopamine receptors and exhibits minimal cross-reactivity with the D2S short receptor.
Catalogue Number324393
Brand Family Calbiochem®
References
ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
Product Information
FormLyophilized
FormulationUndiluted serum.
Positive controlRat brain
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesFrozen Sections Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunohistochemistry
Application NotesELISA (see comments)
Immunoblotting (1:800)
Frozen Sections (1:5000, see application references)
Application CommentsThis antibody is highly specific for the immunizing peptide sequence and is suitable for detection of both the D2S and D2L dopamine receptors in human and rat samples. This antibody has also been reported to work for ELISA. For immunoblotting, a 1:800 dilution of the reconstituted antiserum detects two bands at ~48 and ~51 kDa in an analysis using whole rat brain homogenates. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For frozen sections, a 1:5000 dilution of the reconstituted antiserum demonstrates specific staining of rat brain sections including the plasma membrane in the cortex, caudate putamen, globus pallidus, thalamic nucleus, supra optic nucleus, and the dentate gyrus. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic peptide (CAARRAQELEME) corresponding to amino acids 272-282 of human D₂ dopamine receptor
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Global Trade Item Number
Número de referencia GTIN
324393-100UL 04055977216523