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MABS1805 Anti-Dach1 Antibody, clone 1F6.1

Anti-Dach1, clone 1F6.1, Cat. No. MABS1805, is a mouse monoclonal antibody that detects Dach1 in human and murine cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Dach1 Antibody, clone 1F6.1: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Signaling Gene Symbol: DACH1, DACH UniProt Number: Q9UI36
MABS1805
100 μg  
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MIH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1805
DescriptionAnti-Dach1 Antibody, clone 1F6.1
Alternate Names
  • Dachshund homolog 1
Background InformationDachshund homolog 1 (UniProt: Q9UI36; also known as Dach1) is encoded by the DACH1 (also known as DACH) gene (Gene ID: 1602) in human. Dach1 is a transcription factor that is involved in regulation of organogenesis. Four isoforms of Dach1 have been reported that are produced by alternative splicing. It is shown to be widely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart and isoform 4 is found in spleen. Clone 1F6.1 exhibits reactivity with all four isoforms. Dach1 is reported to act as regulator of SIX1, SIX6, and probably SIX5. It also acts as a a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Dach1 is shown to Inhibit TGF-beta signaling through interaction with SMAD4 and NCOR1. It binds to chromatin DNA via its DACHbox-N domain, which forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain. PELP1 (proline, glutamic acid, and leucine rich protein 1) serves as a Dach1-binding protein and the carboxyl terminus of Dach1 is required for binding to PELP1. Dach1 is reported to prevent the proliferation of cancerous cells due to being a natural repressor of estrogen receptors (ER) in the normal function of mammary tissue. It inhibits ER alpha transcriptional activity. (Ref.: Popov, VM et al. (2009). Cancer Res. 69(14); 5752-60). +D1
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Dach1, clone 1F6.1, Cat. No. MABS1805, is a mouse monoclonal antibody that detects Dach1 in human and murine cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Dach1 in human bone marrow and human colon cancer tissues.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Dach1 in Neuro-2a cell lysate.
Biological Information
ImmunogenGST/His-tagged recombinant fragment coreesponding to 60 amino acids from the C-termnal region of human Dach1.
Clone1F6.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 1F6.1 detects all isoforms of Dach1 in human and murine cells. It targets an epitope within 60 amino acids from the C-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • DACH1
  • DACH
Purification MethodProtein G purified
UniProt Number
Molecular Weight~78 kDa observed; 78.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in SHSY-5Y cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Dach1 in 10 µg of SHSY-5Y cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABS1805 04054839362934