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ABC481 Anti-Cytosolic 5'-nucleotidase II Antibody

Anti-Cytosolic 5'-nucleotidase II Antibody is an antibody against Cytosolic 5'-nucleotidase II for use in Western Blotting, Immunohistochemistry.

Anti-Cytosolic 5'-nucleotidase II Antibody : Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: NT5C2, NT5B, NT5CP, PNT5 UniProt Number: P49902
ABC481
100 μg  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC481
DescriptionAnti-Cytosolic 5'-nucleotidase II Antibody
Alternate Names
  • Cytosolic purine 5'-nucleotidase
  • Cytosolic 5'-nucleotidase II
Background InformationCytosolic purine 5'-nucleotidase (EC 3.1.3.5; UniProt P49902; also known as 5'-nucleotidase (purine), cytosolic type B, Cytosolic 5'-nucleotidase II, IMP-specific 5'-NT, Purine 5' nucleotidase) is encoded by the NT5C2 (also known as cN-II, GMP, NT5B, NT5CP, PNT5, SPG45, SPG65) gene (Entrez Gene ID 22978) in human. NT5C2 protein is a cytosolic enzyme that catalyzes the hydrolysis of inosine 5'-monophosphate (IMP) and other purine nucleotides into the corresponding ribonucleoside and a phosphate. Purine nucleotides are neuroprotective and play a critical role in the ischemic and developing brain, alterations in purine nucleotides levels could sensitize neurons to stress and insult. Hereditary spastic paraplegias (HSPs) are neurodegenerative motor neuron diseases characterized by progressive age-dependent loss of corticospinal motor tract function. Mutations in NT5C2 and several other genes coding for purine nucleotide metabolizing enzymes are linked to autosomal recessive HSP (AR-HSP) in a study of 55 families displaying AR-HSP (PMID 24482476).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-Cytosolic 5'-nucleotidase II Antibody is an antibody against Cytosolic 5'-nucleotidase II for use in Western Blotting, Immunohistochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Cytosolic 5'-nucleotidase II in human kidney tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Cytosolic 5'-nucleotidase II near the C-terminus.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Feline, Canine, Bovine, and Horse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NT5C2
  • NT5B
  • NT5CP
  • PNT5
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~65 kDa observed. This antibody is expected to detect both isoforms.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cytosolic 5'-nucleotidase II in 10 µg of A549 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
ABC481 04055977168921