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MABS2036 Anti-Cytochrome b Antibody, clone 5B3-6E3

Anti-Cytochrome b, clone 5B3-6E3, Cat. No. MABS2036, is a mouse monoclonal antibody that detects Complex III Cytochrome b and has been tested for use in Western Blotting.

Anti-Cytochrome b Antibody, clone 5B3-6E3: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Signaling Gene Symbol: MT-CYB, COB, CYTB, MTCYB UniProt Number: P00156
MABS2036
100 μg  
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS2036
DescriptionAnti-Cytochrome b Antibody, clone 5B3-6E3
Alternate Names
  • Cytochrome b (UniProt: P00156
  • also known as Complex III subunit 3
  • Complex III subunit III
  • Cytochrome b-c1 complex subunit 3
  • Ubiquinol-cytochrome-c reductase complex cytochrome b subunit
Background InformationCytochrome b (UniProt: P00156; also known as Complex III subunit 3, Complex III subunit III, Cytochrome b-c1 complex subunit 3, Ubiquinol-cytochrome-c reductase complex cytochrome b subunit) is encoded by the MT-CYB (also known as COB, CYTB, MTCYB) gene (Gene ID: 4519) in human. Cytochrome b is a mitochondrial inner membrane protein that is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex). It is a part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c and contributes to the generation of a proton gradient across the mitochondrial membrane, which is used for ATP synthesis. The MT-CYB gene is a good phylogenetic marker. Defects in MT-CYB gene are a rare cause of mitochondrial dysfunction underlying different myopathies, which include mitochondrial encephalomyopathy, hypertrophic cardiomyopathy, and sporadic mitochondrial myopathy. Some cases of Leber hereditary optic neuropathy (LHON) are also linked to defects in MT-CYB gene, which result in acute or subacute loss of central vision, due to optic nerve dysfunction.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing HEPES-Buffered Saline (150 mM NaCl, 15 mM HEPES, pH 7.2) with 0.02% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Cytochrome b, clone 5B3-6E3, Cat. No. MABS2036, is a mouse monoclonal antibody that detects Complex III Cytochrome b and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 0.1 µg/mL from a representative lot detected Cytochrome b in mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA. (Courtesy of Michael F. Marusich, Ph.D., mAbDx, Inc., Eugene, OR USA)
Biological Information
ImmunogenKLH-conjugated Synthetic peptide corresponding to the N-terminus of human cytochrome b.
EpitopeN-terminus
Clone5B3-6E3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5B3-6E3 detects cytochrome b in human mitochondria. It targets an epitope within the N-terminal region.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MT-CYB
  • COB
  • CYTB
  • MTCYB
Purification MethodProtein L
UniProt Number
Molecular Weight~28 kDa observed; 42.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected Cytochrome b in Mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABS2036 04054839355578