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ABN289 Anti-Cullin-5 (CUL5) Antibody

Detect Cullin using this rabbit polyclonal antibody, Anti-Cullin-5 (CUL5) Antibody validated for use in western blotting & IHC.

Anti-Cullin-5 (CUL5) Antibody: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: CUL5, VACM1 UniProt Number: Q93034
ABN289
100 µg  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN289
ReplacesAB10622
DescriptionAnti-Cullin-5 (CUL5) Antibody
Alternate Names
  • Cullin-5
  • CUL-5
  • Vasopressin-activated calcium-mobilizing receptor 1
  • VACM-1
Background InformationCUL-5/VACM-1 (cullin-5, vasopressin-activated calcium mobilizing receptor 1) is one component of the ECS (elongin B/C-cul2/cul5-SOCS box protein) complex that forms an E3 ubiquitin ligase that covalently binds ubiquitin to proteins targeted for degradation via the proteasome. CUL-5 is ubiquitously expressed in the central nervous system and in many peripheral organs. Human immunodeficiency virus-1 (HIV-1) protein Vif forms a Skp1-cullin-F-box (SCF)-like complex with CUL-5/VACM-1 and Elongins B and C. The ability of Vif to suppress the antiviral activity of APOBEC3G, which induces hypermutations in newly synthesized viral DNA, is specifically dependent on CUL-5-SCF function, as it allows Vif to interact with APOBEC3G and induce its ubiquitination and degradation. The CUL-5-SCF pathway used by Vif is thus a potential target for antiviral development. CUL-5/VACM-1 inhibits cellular growth by a mechanism that involves MAPK and p53 signaling pathways. In human tissues expression in kidney, brain, heart, placenta, and skeletal muscle is observed. CUL-5/VACM-1 is also expressed in both breast epithelial cells and breast cancer cells. Decreased expression of CUL-5/VACM-1 may play a role in breast tumorigenesis. Further evidence for this finding is observed in T47D breast cancer cells in which overexpression of CUL-5/VACM-1 significantly attenuates cellular proliferation and MAPK phosphorylation when compared to control cells.
References
Product Information
FormatAffinity Purified
Control
  • Human heart tissue lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationDetect Cullin using this rabbit polyclonal antibody, Anti-Cullin-5 (CUL5) Antibody validated for use in western blotting & IHC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Cullin-5 in human placenta and human testis tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Cullin-5 near the Near N-Terminus.
EpitopeNear N-Terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes Cullin-5 near the N-Terminus.
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Rabbit based on 100% sequence homology. Other homologies: Feline (93% sequence homology) and Mouse and Rat (87% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CUL5
  • VACM1
Purification MethodAntigen Affinity Purified
UniProt Number
Molecular Weight~90 kDa observed. Neddylation of Cullin-5 at a.a. 724 with NEDD8 results in a 10 kDa increase in molecular weight (Hori. T., et al. (1999). Oncogene. 18(48):6829-6834).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human heart tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cullin-5 in 10 µg of human heart tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
ABN289 04053252914690