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204903 Anti-Complement 5b-9 Rabbit pAb

204903
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
204903-1MG Ampolla de plást. 1 mg
Description
OverviewRecognizes complement component complex Cb5-C9.
Catalogue Number204903
Brand Family Calbiochem®
SynonymsAnti-Terminal Complement Complex, Anti-Membrane Attack Complex, Anti-MAC
References
ReferencesSchafer, H., et al. 1986. J. Immunol. 137, 1945.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesFrozen Sections Niculescu, F., et al. 2005. Exp. Mol. Pathol. 79, 136.
Key Applications Frozen Sections
Immunofluorescence
Paraffin Sections
Pagelet <collection_feature_application_id-RID> no encontrado.
Application NotesFrozen Sections (see application references)
Immunofluorescence (see comments)
Paraffin Sections (see comments, heat pre-treatment required)
Radial Immunodiffusion (use undiluted)
Application CommentsBy immunodiffusion the antibody is monospecific for C5b-9 complex in purified form or present in cobra venom factor activated human serum. There is no reactivity vs. non-activated normal human serum or plasma. Also reported to work for immunofluorescence. This antibody has been shown to work for formalin-fixed paraffin sections; use of citrate buffer and a pressure cooker for 1 min is required for antigen retrieval. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified human SC5b-9 complex
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing intial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
204903-1MG 04055977219876

Documentation

Anti-Complement 5b-9 Rabbit pAb Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-Complement 5b-9 Rabbit pAb Certificados de análisis

CargoNúmero de lote
204903

Referencias bibliográficas

Visión general referencias
Schafer, H., et al. 1986. J. Immunol. 137, 1945.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-December-2007 JSW
SynonymsAnti-Terminal Complement Complex, Anti-Membrane Attack Complex, Anti-MAC
ApplicationFrozen Sections (see application references)
Immunofluorescence (see comments)
Paraffin Sections (see comments, heat pre-treatment required)
Radial Immunodiffusion (use undiluted)
DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the C5b-C9 complement component complex.
BackgroundC5b-9 is the cytolytically active glycoprotein complex, comprised of C5b, C6, C7, C8 and C9 complement proteins, formed as a result of complement activation via either the classical or alternative pathways.
HostRabbit
Immunogen speciesHuman
Immunogenpurified human SC5b-9 complex
IsotypeIgG
Specieshuman, mouse
FormLiquid
FormulationIn PBS, pH 7.2.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsBy immunodiffusion the antibody is monospecific for C5b-9 complex in purified form or present in cobra venom factor activated human serum. There is no reactivity vs. non-activated normal human serum or plasma. Also reported to work for immunofluorescence. This antibody has been shown to work for formalin-fixed paraffin sections; use of citrate buffer and a pressure cooker for 1 min is required for antigen retrieval. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing intial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesSchafer, H., et al. 1986. J. Immunol. 137, 1945.
Application referencesFrozen Sections Niculescu, F., et al. 2005. Exp. Mol. Pathol. 79, 136.