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ABS2183 Anti-Coagulation factor VIIa

Anti-Coagulation factor VIIa Antibody, Cat. No. ABS2183, is a rabbit polyclonal antibody that detects Coagulation factor VIIa and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.

ABS2183
100 μg  
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue NumberABS2183
DescriptionAnti-Coagulation factor VIIa
Alternate Names
  • Coagulation factor VII
  • EC: 3.4.21.21
  • Proconvertin
  • Serum prothrombin conversion accelerator
  • SPCA
Background InformationCoagulation factor VII (UniProt: P08709; also known as EC: 3.4.21.21, Proconvertin, Serum prothrombin conversion accelerator, SPCA) is encoded by the F7 gene (Gene ID: 2155) in human. Coagulation factor VII is a member of the peptidase S1 family that initiates the extrinsic pathway of blood coagulation. It is serine protease that is synthesized with a signal peptide (aa 1-20) and a propeptide (aa 21-60) and circulates in the blood in a zymogen form. The mature form is a heterodimer of a light and a heavy chain. It is a vitamin K-dependent protease that binds to calcium following enzymatic carboxylation of some glutamate residues. Factor VII is converted to Factor VIIa by the action of Factor Xa, Factor XIIa, Factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, Factor VIIa converts factor X to factor Xa by cleavage of Arg-|-Ile bond. Factor VIIa also converts factor IX to factor IXa in the presence of tissue factor and calcium. Factor VII deficiency resulting from mutations in F7 gene can lead to hemorrhagic complications.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Coagulation factor VIIa Antibody, Cat. No. ABS2183, is a rabbit polyclonal antibody that detects Coagulation factor VIIa and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunofluorescence
  • Western Blotting
Application NotesImmunofluorescence Analysis: A representative lot detected Coagulation factor VIIa in endothelial cell cultures incubated with rFVIIa (6 mg/mL) for 2 h at 37˚C.

Immunocytochemistry Analysis: A representative lot detected Coagulation factor VIIa in umbilical cord veins.
Biological Information
ImmunogenFull-length human recombinant Coagulation factor VIIa.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects coagulation factor VIIa in human.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with other primates based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • F7
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~57 kDa observed; 51.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human placental tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Coagulation factor VIIa in 10 µg of human placental tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
ABS2183 04054839330643

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