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06-503-I Anti-Caspase 1 Antibody

Anti-Caspase 1 Antibody is a highly specific rabbit polyclonal antibody, that targets Caspase 1 & has been tested in western blotting & IHC (Paraffin).

Anti-Caspase 1 Antibody: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Apoptosis & Cancer Research Sub-Category: Caspases Gene Symbol: CASP1, IL1BC, IL1BCE UniProt Number: P29466
06-503-I
100 µg  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number06-503-I
Replaces06-503
DescriptionAnti-Caspase 1 Antibody
Alternate Names
  • Caspase-1
  • CASP-1
  • Interleukin-1 beta convertase
  • IL-1BC
  • Interleukin-1 beta-converting enzyme
  • ICE
  • IL-1 beta-converting enzyme
  • p45
Background InformationCaspase-1, also known as IL-1 beta-converting enzyme (ICE), is an enzyme that cleaves the inflammatory cytokines IL-1 beta and IL-18, releasing the mature, active peptides. Caspase-1 is important for defense against pathogens and it also is involved in the induction of apoptosis. Thymocytes from caspase-1 deficient mice are resistant to Fas-mediated apoptosis, and caspase-1 is similar to the cell death gene CED-3 of C. elegans. Caspase-1 is a heterotetramer that consists of two anti-parallel heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The enzyme has a preferred cleavage site of Tyr-Val-Ala-Asp (YVAD). Caspase-1 is highly expressed in spleen and lung, and is absent from brain.
References
Product Information
FormatAffinity Purified
Control
  • THP cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Caspase 1 Antibody is a highly specific rabbit polyclonal antibody, that targets Caspase 1 & has been tested in western blotting & IHC (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Caspase 1 in human spleen tissue. Additionally, a 1:4,000 dilution from a representative lot detected Caspase 1 in human colon tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the p20 subunit of human Caspase 1.
Epitopep20 subunit
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the p20 subunit of Caspase 1. This antibody may cross-react with Caspase 5 due to 92% sequence homology. Additionally, this antibody recognizes the P45 proenzyme (~45 kDa) and the p20 subunit (~20 kDa) of the reduced, human Caspase 1 isoforms.
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human. Other homologies: Mouse, rat, canine, porcine and feline. (92% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CASP1
  • IL1BC
  • IL1BCE
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~45 kDa observed. Five isoforms between 10-45 kDa are known to exist. This antibody recognizes the P45 proenzyme (~45 kDa) and the p20 subunit (~20 kDa) of the reduced, human Caspase 1 isoforms.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 5 µg/mL of this antibody detected Caspase 1 in 10 µg THP-1 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
06-503-I 04053252882869

Documentation

Anti-Caspase 1 Antibody Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-Caspase 1 Antibody Certificados de análisis

CargoNúmero de lote
Anti-Caspase 1 - 2379496 2379496
Anti-Caspase 1 - 2420548 2420548
Anti-Caspase 1 - 2436803 2436803
Anti-Caspase 1 - 2445063 2445063
Anti-Caspase 1 - 2494275 2494275
Anti-Caspase 1 - 2512058 2512058
Anti-Caspase 1 - 3184725 3184725
Anti-Caspase 1 - 3462121 3462121
Anti-Caspase 1 - 3729455 3729455
Anti-Caspase 1 - 3926374 3926374
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