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04-047 Anti-CUGBP2 Antibody, clone 1H2

This Anti-CUGBP2 Antibody, clone 1H2 is validated for use in WB for the detection of CUGBP2.

Anti-CUGBP2 Antibody, clone 1H2: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Protein Trafficking Research Sub-Category: RNA Metabolism & Binding Proteins, RNA Binding Protein (RBP) Gene Symbol: CUGBP2, CUG-BP2, BRUNOL3, CELF2, CELF-2, Etr-3, ETR-3, ETR3, NAPOR-2, NAPOR2, NAPOR, hNAPOR UniProt Number: O95319
04-047
100 µg  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue Number04-047
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-CUGBP2 Antibody, clone 1H2
References
Product Information
FormatPurified
Presentation100 μg of Protein A purified mouse monoclonal IgG1 in 100μl of 1X PBS, pH 7.0, 0.1% Azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-CUGBP2 Antibody, clone 1H2 is validated for use in WB for the detection of CUGBP2.
Key Applications
  • Western Blotting
Biological Information
ImmunogenA synthetic peptide corresponding to amino acids 209-415 of human CuGBP2.
Cloneclone 1H2
HostMouse
SpecificityCuGBP2
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryMembers of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms.
Gene Symbol
  • CUGBP2
  • CUG-BP2
  • BRUNOL3
  • CELF2
  • CELF-2
  • Etr-3
  • ETR-3
  • ETR3
  • NAPOR-2
  • NAPOR2
  • NAPOR
  • hNAPOR
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O95319 # RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre- mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury (By similarity). Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA (By similarity). Binds to an intronic RNA element responsible for the silencing of exon 21 splicing (By similarity). Binds to (CUG)n repeats (By similarity).


SIZE: 508 amino acids; 54285 Da

SUBUNIT: Found in a complex with APOBEC1 and ACF.

SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Accumulates in the cytoplasm after ionizing radiation (By similarity). Colocalizes with APOBEC1 and ACF. RNA-binding activity is detected in both nuclear and cytoplasmic compartments.

TISSUE SPECIFICITY: Expressed in frontal cortex. Isoform 1 is expressed in brain and lung. Isoform 2 is expressed in heart, brain, placenta, lung, liver, kidney, skeletal muscle and pancreas. Isoform 4 is expressed in heart, lung, skeletal muscle, kidney and pancreas.
DEVELOPMENTAL STAGE: Isoform 1 is expressed in fetal brain. Isoform 2 is expressed in fetal heart, brain, thymus, lung, liver, skeletal muscle, kidney and spleen. Isoform 4 is expressed in fetal heart, brain, thymus, lung and skeletal muscle.




SIMILARITY: SwissProt: O95319 ## Belongs to the CELF/BRUNOL family. & Contains 3 RRM (RNA recognition motif) domains.
Molecular Weight56 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions2 years at -20°C from date of shipment
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
04-047 04053252581625

Documentation

Anti-CUGBP2 Antibody, clone 1H2 Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-CUGBP2 Antibody, clone 1H2 Certificados de análisis

CargoNúmero de lote
Anti-CuGBP2, clone 1H2 (Mouse monoclonal IgG1) - 2433335 2433335
Anti-CuGBP2, clone 1H2 (Mouse monoclonal IgG1) 3066213
Anti-CuGBP2, clone 1H2 (Mouse monoclonal IgG1) - 2074888 2074888
Anti-CuGBP2, clone 1H2 - 3783547 3783547
Anti-CuGBP2, clone 1H2 - DAM1460230 DAM1460230
Anti-CuGBP2, clone 1H2 - DAM1817683 DAM1817683
Anti-CuGBP2, clone 1H2 - JBC1913123 JBC1913123
Anti-CuGBP2, clone 1H2 -2849462 2849462
Anti-CuGBP2, clone 1H2_2830872 2830872

Referencias bibliográficas

Visión general referenciasPub Med ID
ETR-3 and CELF4 protein domains required for RNA binding and splicing activity in vivo
Singh, Gopal, et al
Nucleic Acids Res, 32:1232-41 (2004)  2004

14973222 14973222
Dynamic antagonism between RNA-binding protein CUGBP2 and cyclooxygenase-2-mediated prostaglandin E2 in radiation damage
Murmu, Nabendu, et al
Proc Natl Acad Sci USA, 101:13873-8 (2004)  2004

15358864 15358864
CUGBP2 plays a critical role in apoptosis of breast cancer cells in response to genotoxic injury
Mukhopadhyay, Debnath, et al
Ann N Y Acad Sci, 1010:504-9 (2003)  2003

15033780 15033780
Expression and mutation analysis of BRUNOL3, a candidate gene for heart and thymus developmental defects associated with partial monosomy 10p
Lichtner, P, et al
J Mol Med, 80:431-42 (2002)  2002

12110949 12110949

Folleto

Cargo
RNA-Binding Protein Immunoprecipitation