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MABF2034 Anti-CD31/PECAM-1.2 Antibody, clone MBC 78.2

Anti-CD31/PECAM-1.2, clone MBC 78.2, Cat. No. MABF2034, is a mouse monoclonal that detects PECAM-1 and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Agonist or Inhibitor function, and Western Blotting.

Anti-CD31/PECAM-1.2 Antibody, clone MBC 78.2: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Inflammation & Immunology Gene Symbol: PECAM1 UniProt Number: P16284
MABF2034
100 μg  
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, FC, ICC, IP, AI, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF2034
DescriptionAnti-CD31/PECAM-1.2 Antibody, clone MBC 78.2
Alternate Names
  • Platelet endothelial cell adhesion molecule
  • PECAM-1
  • EndoCAM
  • GPIIA
  • PECA1
  • CD31
Background InformationPlatelet endothelial cell adhesion molecule (UniProt: P16284; also known as PECAM-1, EndoCAM. GPIIA', PECA1, CD31) is encoded by the PECAM1 gene (Gene ID: 5175) in human. PECAM-1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved to generate the mature protein with a large extracellular region (aa 28-601) that contains six Ig-like C2-type domains, followed by a transmembrane segment (aa 602-620) and a cytoplasmic tail (aa. 621-738 of isoform Long) that plays an essential role in PECAM-1-mediated cellular signaling. PECAM-1 is essential for leukocyte transendothelial migration (TEM) under most inflammatory conditions. It is expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Six isoforms of PECAM-1 have been described that are produced by alternative splicing. Isoform Long predominates in all tissues examined, whereas Delta 12 isoform is detected only in trachea and isoform Delta14-15 are only detected in lung tissue. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia and U-937 histiocytic lymphoma cell lines. Tyrosine 690 residue plays a critical role in TEM and is required for efficient trafficking of PECAM-1 to and from the lateral border recycling compartment (LBRC) and is also required for the LBRC membrane to be targeted around migrating leukocytes. PECAM-1 also prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Quality LevelMQ100
Applications
ApplicationAnti-CD31/PECAM-1.2, clone MBC 78.2, Cat. No. MABF2034, is a mouse monoclonal that detects PECAM-1 and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Agonist or Inhibitor function, and Western Blotting.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry
  • Immunoprecipitation
  • Agonist or Inhibitor
  • Western Blotting
Application NotesImmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected CD31/PECAM-1.2 in Jurkat cells.

Agonist or Inhibitor Analysis: A representative lot was used in Agonist or Inhibitor applications (Newton-Nash, D.K., et. al. (1999). J Immunol. 163(2):682-8; Reedguist, K.A., et. al. (2000). J Cell Biol. 148(6):1151-8).

Flow Cytometry Analysis: 1 µg from a representative lot detected CD31/PECAM-1.2 in THP-1 cells.

ELISA Analysis: A representative lot detected CD31/PECAM-1.2 in ELISA applications (Mei, H., et. al. (2014). J Biol Chem. 289(30):20836-44).

Flow Cytometry Analysis: A representative lot detected CD31/PECAM-1.2 in Flow Cytometry applications (Newton-Nash, D.K., et. al. (1999). J Immunol. 163(2):682-8).

Immunoprecipitation Analysis: A representative lot detected CD31/PECAM-1.3 in Immunoprecipitation applications (Newman, D.K., et. al. (2016). Sci Signal. 9(418):ra27).

Western Blotting Analysis: A representative lot detected CD31/PECAM-1.3 in Western Blotting applications (Newman, D.K., et. al. (2016). Sci Signal. 9(418):ra27).
Biological Information
ImmunogenPECAM-1 purified from human platelets.
Epitopeextracellular domain
CloneMBC 78.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone MBC 78.2 detects CD31/PECAM-1 in human cells. It targets an epitope within the IgG homology domain 6.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PECAM1
Purification MethodProtein G purified
UniProt Number
Molecular Weight82.54 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in THP-1 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected CD31/PECAM-1.2 in THP-1 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABF2034 04054839360961

Documentation

Anti-CD31/PECAM-1.2 Antibody, clone MBC 78.2 Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-CD31/PECAM-1.2 Antibody, clone MBC 78.2 Certificados de análisis

CargoNúmero de lote
Anti-CD31/PECAM-1.2, clone MBC 78.2 - 3426908 3426908
Anti-CD31/PECAM-1.2, clone MBC 78.2 - 3707332 3707332
Anti-CD31/PECAM-1.2, clone MBC 78.2 - 4073076 4073076
Anti-CD31/PECAM-1.2, clone MBC 78.2 - 4073664 4073664
Anti-CD31/PECAM-1.2, clone MBC 78.2 Monoclonal Antibody Q2933432